Electrochemical magnetoimmunosensing strategy for the detection of pesticides residues

Analytical Chemistry
E ZaccoM-P Marco

Abstract

A novel electrochemical immunosensing strategy for the detection of atrazine based on magnetic beads is presented. Different coupling strategies for the modification of the magnetic beads with the specific anti-atrazine antibody have been developed. The immunological reaction for the detection of atrazine performed on the magnetic bead is based on a direct competitive assay using a peroxidase (HRP) tracer as the enzymatic label. After the immunochemical reactions, the modified magnetic beads can be easily captured by a magnetosensor made of graphite-epoxy composite, which is also used as the transducer for the electrochemical immunosensing. The electrochemical detection is thus achieved through a suitable substrate and mediator for the enzyme HRP. The electrochemical approach is also compared with a novel magneto-ELISA based on optical detection. The performance of the electrochemical immunosensing strategy based on magnetic beads was successfully evaluated using spiked real orange juice samples. The detection limit for atrazine using the competitive electrochemical magnetoimmunosensing strategy with anti-atrazine-specific antibody covalent coupled with tosyl-activated magnetic beads was found to be 6 x 10(-3) microg L(-1) (0.0...Continue Reading

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Citations

Feb 21, 2009·Analytical and Bioanalytical Chemistry·Hideki Kuramitz
Jul 28, 2013·Analytical and Bioanalytical Chemistry·Raul Garcia-FebreroM-Pilar Marco
Aug 3, 2013·Analytical and Bioanalytical Chemistry·A MurianoM-P Marco
Sep 28, 2013·Analytica Chimica Acta·Silvina V KergaravatSilvia R Hernandez
May 21, 2013·Biosensors & Bioelectronics·Silvina V KergaravatSilvia R Hernandez
May 22, 2008·Analytical Chemistry·Benjamin J PrivettMark H Schoenfisch
Jan 1, 2009·Sensors·Lia StanciuSilvana Andreescu
Nov 19, 2010·Bioanalysis·Sonia CentiMarco Mascini
Dec 24, 2013·Applied Microbiology and Biotechnology·Tamara LaubeMaría Isabel Pividori
Jun 15, 2016·Biosensors & Bioelectronics·E Sánchez-TiradoJ M Pingarrón
Feb 15, 2013·Analytical Chemistry·Susana LiébanaMaría Isabel Pividori
Jan 13, 2009·Journal of Environmental Monitoring : JEM·Silvana AndreescuMatthew T Ravalli
Nov 14, 2015·Critical Reviews in Food Science and Nutrition·Begoña Martín-FernándezBeatriz López-Ruiz
Jan 6, 2019·Analytical and Bioanalytical Chemistry·Enrique ValeraM-P Marco
Jan 6, 2019·Talanta·Amal H A HassanMaria Isabel Pividori
Nov 21, 2007·Analytical Chemistry·Dolors JornetRosa Puchades
Aug 12, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Thangavelu SelvarajuHaesik Yang
Jun 25, 2021·Analytical and Bioanalytical Chemistry·J MarfàM I Pividori
Jun 21, 2007·Analytical Chemistry·Adriano AmbrosiArben Merkoçi
Oct 7, 2021·Journal of Agricultural and Food Chemistry·Xin ZhaoHongyan Bi

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