Electrochemical microfluidic biosensor for the detection of nucleic acid sequences

Lab on a Chip
Vasiliy N GoralAntje J Baeumner

Abstract

A microfluidic biosensor with electrochemical detection for the quantification of nucleic acid sequences was developed. In contrast to most microbiosensors that are based on fluorescence for signal generation, it takes advantage of the simplicity and high sensitivity provided by an amperometric and coulorimetric detection system. An interdigitated ultramicroelectrode array (IDUA) was fabricated in a glass chip and integrated directly with microchannels made of poly(dimethylsiloxane) (PDMS). The assembly was packaged into a Plexiglas housing providing fluid and electrical connections. IDUAs were characterized amperometrically and using cyclic voltammetry with respect to static and dynamic responses for the presence of a reversible redox couple-potassium hexacyanoferrate (ii)/hexacyanoferrate (iii) (ferri/ferrocyanide). A combined concentration of 0.5 microM of ferro/ferricyanide was determined as lower limit of detection with a dynamic range of 5 orders of magnitude. Background signals were negligible and the IDUA responded in a highly reversible manner to the injection of various volumes and various concentrations of the electrochemical marker. For the detection of nucleic acid sequences, liposomes entrapping the electrochemica...Continue Reading

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Citations

Nov 17, 2006·Analytical and Bioanalytical Chemistry·Ingrid Fritsch, Zoraida P Aguilar
Mar 11, 2008·Analytical and Bioanalytical Chemistry·Katie A EdwardsAntje J Baeumner
Sep 9, 2008·Analytical and Bioanalytical Chemistry·Natinan BunyakulAntje J Baeumner
Sep 13, 2011·Analytical and Bioanalytical Chemistry·John T ConnellyAntje J Baeumner
Oct 1, 2011·Analytical and Bioanalytical Chemistry·John T Connelly, Antje J Baeumner
May 18, 2013·Analytical and Bioanalytical Chemistry·Nongnoot WongkaewAntje J Baeumner
Sep 12, 2008·Applied Microbiology and Biotechnology·Daniel PiochBritta Jürgen
Feb 20, 2013·Biomedical Microdevices·Jiyong HuangMinhee Yun
Apr 18, 2012·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Shaurya PrakashBharat Bhushan
Aug 21, 2009·Biomicrofluidics·Satyajyoti SenapatiHsueh-Chia Chang
Dec 18, 2013·Analytical Chemistry·Sarah J ReinholtAntje J Baeumner
Oct 2, 2014·The Analyst·Sahana SarkarSerge G Lemay
Mar 9, 2012·Lab on a Chip·E Victoria DydekKlavs F Jensen
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Jun 22, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Ranjan Ganguly, Ishwar K Puri
Jan 3, 2006·Biosensors & Bioelectronics·Sylvia KwakyeAntje J Baeumner
Jan 31, 2008·Lab on a Chip·Daniel BerdatMartin A M Gijs
Nov 18, 2011·Lab on a Chip·Azadeh NilghazMohammed Rafiq Abdul Kadir
Oct 18, 2012·The Analyst·Qingtao Liu, Ben J Boyd
Nov 20, 2013·Nanoscale·Martin HüskeBernhard Wolfrum
Jun 25, 2014·Analytical and Bioanalytical Chemistry·Natinan BunyakulAntje J Baeumner
Jan 27, 2009·Biosensors & Bioelectronics·Sam R NugenAntje J Baeumner
May 20, 2016·Journal of Biomaterials Applications·Tohid Mahmoudi-BadikiSeyed Mahdi Golabi
Mar 27, 2020·Micromachines·Jiye FuZuhong Lu
Oct 22, 2019·Advanced Functional Materials·Nitinun VarongchayakulMark Grinstaff
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Sep 8, 2016·Micromachines·Ahmad Rezaei KolahchiAmir Sanati-Nezhad
Dec 10, 2020·The Analyst·Cornelia A HermannAntje J Baeumner
Mar 16, 2021·Emergent Materials·Sultan ŞahinLevent Trabzon
Dec 8, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Chih-Hsin Chen, Kun-Lin Yang

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