Electrochemical Monitoring of the Reversible Folding of Surface-Immobilized DNA i-Motifs

Langmuir : the ACS Journal of Surfaces and Colloids
Catherine AdamThomas Doneux

Abstract

Two cytosine (C) rich DNA sequences folding in i-motif upon protonation of C at low pH have been immobilized at gold electrodes to study the impact of the electrode|electrolyte interface on the stability of the noncanonical DNA secondary structure. The effects of the molecular composition and environment on the melting and folding of the structures immobilized at the gold surface have been compared to the properties of the DNA strands in solution. The DNA folding into i-motif upon protonation, both at the surface and in solution, results in a significant variation of the charge density which is monitored electrochemically through the electrostatic interactions between the DNA strand and the electroactive hexaammineruthenium(III). This method is shown to be sufficiently sensitive to distinguish hemiprotonated folded state and single strand unfolded state of i-motif. The pH of melting has been determined for both sequences in the bulk and at the gold|electrolyte interface. The results evidence a stabilizing effect of the interface on i-motif structure, whereby the pH of melting is higher for the sequences immobilized at the surface. The reversibility and precision of the electrochemical model described here allows a clear and sim...Continue Reading

References

Jan 1, 1982·Methods in Enzymology·K J Ellis, J F Morrison
Dec 9, 1998·Analytical Chemistry·A B SteelM J Tarlov
Nov 1, 2002·Nucleic Acids Research·Anh Tuân Phan, Jean-Louis Mergny
Jan 8, 2004·Journal of the American Chemical Society·Daisuke MiyoshiNaoki Sugimoto
Sep 15, 2005·Nucleic Acids Research·Jean-Louis MergnyJonathan B Chaires
Apr 19, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Ian BurgessR Bruce Lennox
Jun 29, 2006·Journal of the American Chemical Society·Gang ShenRastislav Levicky
Nov 14, 2008·Journal of the American Chemical Society·Sumeet MahajanPhilip N Bartlett
Sep 3, 2011·Journal of the American Chemical Society·Jungkweon ChoiTetsuro Majima
Sep 9, 2011·Chemical Society Reviews·Jungkweon Choi, Tetsuro Majima
Jul 28, 2012·Journal of the American Chemical Society·Robert P JohnsonPhilip N Bartlett
May 23, 2014·Accounts of Chemical Research·Yuanchen DongDongsheng Liu
Jul 23, 2014·Analytical Chemistry·Aurore De RacheClaudine Buess-Herman
Jul 25, 2017·The Journal of Physical Chemistry. B·Tra NguyenRichard D Sheardy
Dec 13, 2017·Physical Chemistry Chemical Physics : PCCP·Weibin RenJiang Zhao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved