Electrochemical probing of HIV enzymes using ferrocene-conjugated peptides on surfaces

The Analyst
Kagan Kerman, Heinz-Bernhard Kraatz

Abstract

One of the current pathways to develop inhibitors that target different steps in the life cycle of the human immunodeficiency virus (HIV) is blocking the function of the HIV-related proteins such as HIV-1 integrase (HIV-1 IN), HIV-1 reverse transcriptase (HIV-1 RT) and HIV-1 protease (HIV-1 PR), which are essential proteins that control the ability of HIV to infect cells, produce new copies of the virus, or cause disease. We have demonstrated for the first time the detection of these enzymes at nanomolar levels using ferrocene (Fc)-conjugated peptides on gold microelectrodes. The interaction between the Fc-conjugated peptides and the enzymes was studied by cyclic voltammetry. As the protein concentration increased, the electrochemical behaviour of the surface-bound Fc- bioconjugate changed, indicating that HIV protein was binding to the peptide film and encapsulating the Fc redox center on the surface. The electrochemical responses shifted to higher potentials and decreased in the current intensity, as the concentrations of the HIV-1 enzymes increased. The optimization studies were performed by changing the pH and NaCl concentration. Control experiments involved the exposure of the Fc-conjugated peptides with all the enzymes. T...Continue Reading

References

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Citations

Nov 8, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Daniel J FeldThomas J Meade
Mar 29, 2014·Chemical Communications : Chem Comm·Adam McQuistanRebecca Y Lai
Jun 15, 2014·Analytica Chimica Acta·Kazuharu SugawaraShunitz Tanaka
Apr 13, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Sanela MartićHeinz-Bernhard Kraatz
Jan 31, 2012·The Analyst·Modupeola A Sowole, Heinz-Bernhard Kraatz
Dec 14, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Samaneh BeheshtiHeinz-Bernhard Kraatz
Apr 10, 2010·Accounts of Chemical Research·Toshiyuki Moriuchi, Toshikazu Hirao
Oct 31, 2009·Journal of the American Chemical Society·Libo HuKevin D Moeller

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