PMID: 6968864Jan 1, 1980Paper

Electrochemical profile for ion transport across the membrane of proximal tubular cells

Membrane Biochemistry
M FujimotoT Kubota

Abstract

A micropuncture study was performed on the bullfrog kidney proximal tubular cells utilizing double-barreled ion-selective microelectrodes. The intracellular of Na+, K+, Cl-, HCO3(-) and pH were determined to be 21.6 mEq/L, 67.4 mEq/L, 9.9 mEq/L, 20.2 mEq/L, and 7.49 pH units, respectively. In the extracellular fluid the following activities were found: Na+, 87.4 mEq/L; K+, 2.64 mEq/L; Cl-, 72.5 mEq/L; HCO3(-), 17.9 mEq/L; and pH, 7.66. The membrane potential difference was 68.4 mV and 60.4 mV across the peritubular and brush borders, respectively. The electrochemical potential differences across the individual borders of the proximal tubule cells were separately calculated by setting the intracellular level of both electrical and chemical potentials at zero for convenience. From these analyses, the following interpretations are made. (1) In the net reabsorption of Na+, luminal Na+ enters the cell along a 95-mV gradient across the luminal border and is pumped out to the interstitium against a 104 mV gradient. In the reabsorption of bicarbonate, an uphill pump of about 69 mV (about 70% of the Na+ entry gradient) must exist on the luminal border, of which about 55 mV (80% of the bicarbonate gradient) is accounted for by the H+ sec...Continue Reading

References

Jan 1, 1978·The Japanese Journal of Physiology·T Kubota, M Fujimoto
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Aug 5, 1975·Biochimica Et Biophysica Acta·W M ArmstrongC J Myers
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Citations

May 1, 1984·Pflügers Archiv : European journal of physiology·R GregerB Gebler
Dec 1, 1983·Pflügers Archiv : European journal of physiology·A C CassolaE Frömter
Jan 1, 1982·The Journal of Membrane Biology·P J Gunter-Smith, S G Schultz
Jun 24, 1985·Neuroscience Letters·D Ammann, P Anker
Aug 1, 1986·Kidney International·G A Quamme, J H Dirks
Aug 17, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ryan M Pelis, J Larry Renfro
May 1, 1989·The American Journal of Physiology·H R BradyS R Gullans

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