PMID: 6986161Jan 8, 1980Paper

Electrochemical proton gradient in inverted membrane vesicles from Escherichia coli

Biochemistry
W W ReenstraH R Kaback

Abstract

Inverted membrane vesicles prepared from Escherichia coli ML 308-225 generate a transmembrane electrochemical proton gradient (delta mu H+; interior positive and acid) during oxidation of D-lactate, succinate, reduced phenazine methosulfate, or NADH or hydrolysis of ATP. Using the distribution of the lipophilic anion thiocyanate to measure the membrane potential (delta psi) and the permeant weak base methylamine to measure the pH gradient (delta pH), maximal values for delta psi H+ of approximately +160 mV are obtained. Many of the properties of delta psi H+ in inverted vesicles are similar to those described previously in right-side-out vesicles [Ramos, S., & Kaback, H.R. (1977) Biochemistry 16, 848]: (1) the magnitude of the delta psi (interior positive) generated in the presence of D-lactate or reduced phenazine methosulfate is similar to that observed in right-side-out vesicles but of opposite polarity and independent of pH from 5.5 to 8.0; (2) plots of delta pH vs. internal pH in the right-side-out vesicles are similar with D-lactate as the electron donor; (3) as observed with right-side-out vesicles, dissipation of delta psi or delta pH leads to a concomitant increase in the other parameter without a change in the rate of...Continue Reading

References

Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·P Stroobant, H R Kaback
Apr 1, 1976·European Journal of Biochemistry·E PadanH Rottenberg
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S RamosH R Kaback
Dec 1, 1976·Journal of Cellular Physiology·H R Kaback
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G NavonT Yamane
Feb 21, 1978·Biochemistry·S Schuldiner, H Fishkes
Apr 11, 1978·Biochimica Et Biophysica Acta·S OgawaG Navon
Apr 15, 1979·Archives of Biochemistry and Biophysics·J C Beck, B P Rosen
Jun 1, 1979·Archives of Biochemistry and Biophysics·A P Singh, P D Bragg
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P Owen, H R Kaback
Apr 17, 1979·Biochemistry·P Owen, H R Kaback
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D LichtshteinA J Blume
Mar 17, 1977·Biochimica Et Biophysica Acta·M EisenbachS R Caplan
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·B P Rosen, J S McClees
May 7, 1974·Biochemical and Biophysical Research Communications·E L Hertzberg, P C Hinkle
Aug 9, 1972·Biochimica Et Biophysica Acta·D W DeamerA R Crofts
Aug 1, 1966·Biological Reviews of the Cambridge Philosophical Society·P Mitchell

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Citations

Nov 2, 1993·Biochimica Et Biophysica Acta·H Rottenberg, R Moreno-Sanchez
Jan 30, 1989·Biochimica Et Biophysica Acta·E Nissani, H Ginsburg
Dec 1, 1981·Biochimica Et Biophysica Acta·E PadanS Schuldiner
Sep 6, 1984·Biochimica Et Biophysica Acta·L DanielsG D Sprott
Jun 3, 1985·Biochimica Et Biophysica Acta·A L DrachevV P Skulachev
Jun 14, 2005·Comptes rendus biologies·H Ronald Kaback
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Oded LewinsonEitan Bibi
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G J KaczorowskiH R Kaback
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H KieferH R Kaback
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J HugenholtzH R Kaback
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T GoldkornH R Kaback
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K MatsushitaH R Kaback
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N CarrascoH R Kaback
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L Chen, P C Tai
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T HamamotoM Montal
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K L KorthC S Levings
Jun 1, 1981·European Journal of Biochemistry·M Michels, E P Bakker
Nov 1, 1981·European Journal of Biochemistry·W C HorneE R Simons
Jul 15, 1983·European Journal of Biochemistry·J L AbrahamsonJ M Wood
Apr 1, 1985·European Journal of Biochemistry·E DamianoG Leblanc
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Lan Guan, H Ronald Kaback
Aug 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lan Guan, H Ronald Kaback
Aug 12, 2014·Nature Communications·Nir FlumanEitan Bibi
Mar 20, 2014·The Journal of Biological Chemistry·Octavian CălinescuKlaus Fendler
Jan 1, 1987·Critical Reviews in Biotechnology·J L MilnerJ M Wood
Apr 26, 2005·Biophysical Chemistry·Marta HakobyanKarine Bagramyan
Feb 15, 1984·Archives of Biochemistry and Biophysics·M G Borbolla, B P Rosen
Aug 14, 2003·Biochemical and Biophysical Research Communications·Nelli MnatsakanyanArmen Trchounian
Jan 1, 1980·Annals of the New York Academy of Sciences·H R Kaback
Jan 1, 1981·Annals of the New York Academy of Sciences·S Scheiner
Jan 1, 1980·Annals of the New York Academy of Sciences·G J KaczorowskiH R Kaback
Mar 13, 1986·Biochimica Et Biophysica Acta·J K Wright
Nov 10, 2005·Biochimica Et Biophysica Acta·Etana PadanTerry A Krulwich
Feb 22, 2012·Biochimica Et Biophysica Acta·Adriana RycovskaHartmut Michel

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