Electrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles

Mikrochimica Acta
Fanjun ZhuWenchao Dou

Abstract

A highly sensitive electrochemical sandwich immunoassay is described for determination of Escherichia coli O157:H7 (E. coli O157:H7). Silica coated magnetite nanoparticles (Fe3O4) were modified with primary antibody to capture E. coli O157:H7. Gold-platinum core/shell nanoparticles (Au@Pt NPs) with different Pt shell thicknesses were prepared via changing the molar ratio of H2PtCl6 to HAuCl4 in the precursor solution. The optimized Au@Pt NPs exhibit enhanced activity in the electrocatalytic reduction of hydrogen peroxide (H2O2). The Au@Pt NPs were modified with graphene that was functionalized with Neutral Red, and then used as an electrochemical label for secondary antibodies and horseradish peroxidase (HRP). The sandwich immunocomplexes were magnetically absorbed on a 4-channel screen printed carbon electrode. Due to the catalysis of the Au@Pt NPs and HRP, the signal is strongly amplified in the presence of H2O2 when using thionine as the electron mediator. Under optimal conditions, the immunoassay has a linear response in the 4.0 × 102 to 4.0 × 108 CFU·mL-1 concentration range, with a limit of detection of 91 CFU·mL-1 (at an S/N ratio of 3). Graphical abstract Preparation of Au@Pt core/shell nanoparticles with different Pt s...Continue Reading

References

Dec 25, 2007·Biotechnology Advances·Liju Yang, Rashid Bashir
Apr 11, 2008·Chemical Communications : Chem Comm·Noel KristianXin Wang
Sep 23, 2014·Biosensors & Bioelectronics·Abdel-Rahim Hussein Abdel-Azzem HassanArben Merkoçi
Oct 30, 2014·Analytical Chemistry·Chengzhou ZhuYuehe Lin

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Citations

Nov 22, 2019·Mikrochimica Acta·Ramin PourakbariBalal Khalilzadeh
Nov 28, 2019·Sensors·Alba Iglesias-MayorAlfredo de la Escosura-Muñiz
Jul 6, 2020·Mikrochimica Acta·Susana CampuzanoJosé M Pingarrón
Sep 15, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Parinaz BabaieMohammad Hasanzadeh
Aug 11, 2020·Biosensors & Bioelectronics·Zixin JiangZhihe Qing
Sep 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Lunjie HuangQingyi Wei
May 4, 2021·Talanta·Elham SheikhzadehMohammed Zourob

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