Electrochemical sensing of membrane potential and enzyme function using gallium arsenide electrodes functionalized with supported membranes

The Journal of Physical Chemistry. B
Daniel GassullMotomu Tanaka

Abstract

We deposit phospholipid monolayers on highly doped p-GaAs electrodes that are precoated with methyl-mercaptobiphenyl monolayers and operate such a biofunctional electrolyte-insulator-semiconductor (EIS) setup as an analogue of a metal-oxide-semiconductor setup. Electrochemical impedance spectra measured over a wide frequency range demonstrate that the presence of a lipid monolayer remarkably slows down the diffusion of ions so that the membrane-functionalized GaAs can be subjected to electrochemical investigations for more than 3 days with no sign of degradation. The biofunctional EIS setup enables us to translate changes in the surface charge density Q and bias potentials Ubias into the change in the interface capacitance Cp. Since Cp is governed by the capacitance of semiconductor space charge region CSC, the linear relationships obtained for 1/Cp2 vs Q and 1/Cp2 vs Ubias suggests that Cp can be used to detect the surface charges with a high sensitivity (1 charge per 18 nm2). Furthermore, the kinetics of phospholipids degradation by phospholipase A2 can also be monitored by a significant decrease in diffusion coefficients through the membrane by a factor of 104. Thus, the operation of GaAs membrane composites established here...Continue Reading

References

Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M Montal, P Mueller
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Sep 16, 2000·Physical Review Letters·C A KellerB Kasemo
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Jun 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dhaval A DoshiJaroslaw Majewski

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Citations

Aug 24, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Fernanda Camacho-AlanisNathan S Swami
Sep 5, 2009·The European Physical Journal. E, Soft Matter·T SchubertM Tanaka
May 7, 2011·Chemistry, an Asian Journal·Motomu TanakaFernanda F Rossetti
Dec 8, 2011·Analytical Chemistry·Irep Gözen, Aldo Jesorka

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