Electrochemical sensing of nuclear matrix protein 22 in urine with molecularly imprinted poly(ethylene-co-vinyl alcohol) coated zinc oxide nanorod arrays for clinical studies of bladder cancer diagnosis

Biosensors & Bioelectronics
Mei-Hwa LeeHung-Yin Lin

Abstract

In 1996 and 2000, the US Food and Drug Administration (FDA) approved the use of Nuclear matrix protein 22 (NMP22) as a monitoring tool for predicting the recurrence/clearing of bladder cancer, and for screening undiagnosed individuals who have symptoms of, or are at risk for, that disease. The fabrication of electrodes for sensing NMP22 and their integration with a portable potentiostat in a homecare system may have great value. This work describes a sensing element comprised of molecularly imprinted polymers (MIPs) for the specific recognition of NMP22 target molecules. Zinc oxide (ZnO) nanorods (214 ± 45 nm in diameter and 1.08 ± 0.11 μm long) were hydrothermally grown on the sensing electrodes to increase the surface area to be coated with MIPs. A portable potentiostat was assembled and a data acquisition (DAQ) card and the Labview program were utilized to monitor electrochemical reaction to sense NMP22 in urine samples. Finally, in phase 0 clinical trials, measurements were made of samples from a few patients with bladder cancer using the NMP22 MIP-coated ZnO nanorods electrodes that were integrated into a portable potentiostat, revealing NMP 22 concentrations in the range 128 ± 19 to 588 ± 53 ng/mL.

References

Oct 26, 2005·Journal of Nanoscience and Nanotechnology·Zhiyong Fan, Jia G Lu
Jun 20, 2006·Biosensors & Bioelectronics·Chung-Yi HsuTse-Chuan Chou
Sep 12, 2006·Biosensors & Bioelectronics·Chun-Yueh HuangBin-Da Liu
Jan 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yusuke FuchiwakiIzumi Kubo
Oct 4, 2007·Organic & Biomolecular Chemistry·Adam McCluskeyMichael C Bowyer
Feb 24, 2009·Biosensors & Bioelectronics·Chun-Yueh HuangHung-Yin Lin
Oct 8, 2013·ACS Applied Materials & Interfaces·Wen-Janq ChenHung-Yin Lin
Nov 12, 2013·Current Molecular Medicine·Y ZhangW Cai
Jan 30, 2014·European Urology·Robert S SvatekYair Lotan
Oct 31, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Mei-Hwa LeeHung-Yin Lin
Aug 19, 2015·Colloids and Surfaces. B, Biointerfaces·Mei-Hwa LeeHung-Yin Lin

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Citations

Jun 30, 2016·Journal of Separation Science·Liyin WenYongqin Lv
Mar 30, 2017·Sensors·Giulia Selvolini, Giovanna Marrazza
Jul 25, 2018·Frontiers in Pharmacology·Ming-Hui HeZhi-Xiang Yuan
Jun 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mei-Hwa LeeHung-Yin Lin
Dec 9, 2020·Biosensors & Bioelectronics·Mei-Hwa LeeHung-Yin Lin
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Katarína Nemčeková, Ján Labuda

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