Electrochemical Sensor Based on Molecularly Imprinted Polymer for the Detection of Cefalexin

Biosensors
Bogdan FeierCecilia Cristea

Abstract

In this study, a new electrochemical sensor was developed for the detection of cefalexin (CFX), based on the use of a molecularly imprinted polymer (MIP) obtained by electro‒polymerization in an aqueous medium of indole-3-acetic acid (I3AA) on a glassy carbon electrode (GCE) and on boron-doped diamond electrode (BDDE). The two different electrodes were used in order to assess how their structural differences and the difference in the potential applied during electrogeneration of the MIP translate to the performances of the MIP sensor. The quantification of CFX was performed by using the electrochemical signal of a redox probe before and after the rebinding of the template. The modified electrode was characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The influence of different parameters on the fabrication of the sensor was tested, and the optimized method presented high selectivity and sensitivity. The MIP-based electrode presented a linear response for CFX concentration range of 10 to 1000 nM, and a limit of detection of 3.2 nM and 4.9 nM was obtained for the BDDE and the GCE, respectively. The activity of the sensor w...Continue Reading

References

Feb 5, 2004·Journal of the American Chemical Society·Biao LiuStephen E Creager
Jun 19, 2008·Chemical Reviews·Richard L McCreery
Oct 22, 2011·International Journal of Molecular Sciences·Giuseppe VasapolloGiuseppe Mele
Dec 19, 2014·Physical Chemistry Chemical Physics : PCCP·Julie V Macpherson
May 2, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Weiqing LiFeng Zhang
Oct 25, 2016·Trends in Biotechnology·Maciej Cieplak, Włodzimierz Kutner
Jun 4, 2017·Analytica Chimica Acta·Bogdan FeierRobert Săndulescu

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Citations

Oct 12, 2019·The Analyst·Olja Simoska, Keith J Stevenson
Oct 7, 2020·Journal of Molecular Recognition : JMR·Ingrid BenachioLuís Moreira Gonçalves
Dec 19, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ai-Yue HaoChuan-Chao Dai
Nov 10, 2020·Biosensors & Bioelectronics·Patrícia RebeloCristina Delerue-Matos
May 1, 2021·Analytical and Bioanalytical Chemistry·Anna Herrera-ChacónManel Del Valle

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Methods Mentioned

BETA
atomic
scanning electron microscopy
AFM

Software Mentioned

Nova

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