Electrochemical Sensor for Detection of miRs Based on the Differential Effect of Competitive Structures in The p19 Function.

Scientific Reports
E GhazizadehS Hosseinkhani

Abstract

The present study aim to design a liposomal electrochemical sensor using 1, 2-dioleoyl-3-trimethylammoniumpropane (DOTAP) and dioleoylphosphatidylethanolamine(DOPE), chimeric probes and p19, it has been considered as a caliper molecule as well. Also the competitor structural hybrid (RNA) was used to detect three types of miRs in one screen printed electrode modified by gold nanoparticle (SCPE/GNP). In this purpose, the sensor signal stabilized when the cationic DOTAP-DOPE with hybrids of the chimeric probes (Stem, M-linear) sandwiched in order to detect 221-124a miRs. Given the lack of accessibility to RNA-miRs segments of chimeric probes, p19 inhibited the electrochemical reaction and shifted signal to off. After that p19 connected with the free hybrid of T-linear/21miR (just RNA) as competing for structure and the signal was shifted to ON, again. In this study, the electrochemical measurements were performed between the potentials at -0.4 V and +0.4 V with 1 mM [Fe(CN)6]-3-/4 which DOTAP-DOPE acted as an enhancer layer in the electrostatically reaction. This sensor determines as low as 0.4 fM of miRNA with high selectivity and specificity for sequential analysis of 124a-221-21 miRs in just 2 h.

References

Mar 5, 2009·Analytical Chemistry·Wei-Ching Liao, Ja-An Annie Ho
Jun 24, 2010·BioTechniques·Jingmin JinLarry A McReynolds
Nov 6, 2012·Biosensors & Bioelectronics·Farangis AtaeiSaman Hosseinkhani
Dec 1, 2012·Cancer Letters·Jun ShenFeng Jiang
Feb 1, 2013·Journal of the American Chemical Society·Mahmoud LabibMaxim V Berezovski
Aug 6, 2013·Analytical Chemistry·Pankaj RamnaniAshok Mulchandani
Sep 24, 2013·Talanta·Ezat Hamidi-AslMarco Mascini
Jan 1, 2011·Cancers·Silvia CatuognoVittorio De Franciscis
Nov 20, 2013·Analytical and Bioanalytical Chemistry·Susana CampuzanoJosé M Pingarrón
Feb 5, 2014·Nature Reviews. Clinical Oncology·Heidi SchwarzenbachKlaus Pantel
Feb 7, 2014·The Analyst·Blake N Johnson, Raj Mutharasan
Nov 13, 2015·The Journal of Physical Chemistry. B·Wei DingMario Orsi
Nov 30, 2016·Biosensors & Bioelectronics·Mojtaba ShamsipurSaman Hosseinkhani

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Citations

Mar 31, 2020·Analytical and Bioanalytical Chemistry·Mohammad Kaji YazdiAli Neshastehriz
Apr 8, 2020·Analytica Chimica Acta·Mohammad Kaji YazdiAli Neshastehriz

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Methods Mentioned

BETA
DropSens
PBASE
AFM
biosensor

Software Mentioned

ZSimpWin

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