Electrochemical tropomyosin allergen immunosensor for complex food matrix analysis

Analytica Chimica Acta
A Angulo-IbáñezS Merino

Abstract

A shrimp tropomyosin (TPM) immunosensor has been developed and optimized to detect trace amounts of shrimp (in the ppm range), based on a combination of an amperometric transduction, magnetic particles and disposable screen-printed electrodes. The approach is based on the implementation of a sandwich immunoassay format on the surface of magnetic beads and their coupling onto disposable screen-printed electrodes to finally register the amperometric response at -200 mV vs. Ag pseudo-reference electrode, using H2O2 as enzymatic substrate and hydroquinone as redox mediator. The use of carboxyl-functionalized magnetic microbeads (MBs) and in-house made magnetic nanoparticles (MNPs) as solid supports have been evaluated and compared. Our experimental results confirm that the use of MBs, in addition to simplifying the test protocol, improves the resulting sensitivity, so they were selected for the implementation of the immunosensor. In the optimized experimental conditions, the developed immunosensor offered a LOD of 47 pg mL-1 for amperometric determination of shrimp TPM standards and great selectivity against TPM from other sources, thus allowing differentiation between crustaceans (shrimp) and mollusks (squid). Applicability studie...Continue Reading

Citations

Dec 3, 2020·Biosensors·Adriano Aquino, Carlos Adam Conte-Junior
Nov 27, 2020·Journal of AOAC International·Tomasz Tuzimski, Anna Petruczynik
Apr 9, 2021·Critical Reviews in Food Science and Nutrition·Jiayuan XuXiulan Sun
Jun 2, 2021·Critical Reviews in Food Science and Nutrition·Sara BenedéElena Molina
Jun 30, 2021·Critical Reviews in Food Science and Nutrition·Jilin LiJun-Hu Cheng

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