Electrochemically Synthesized Silver Nanoparticles Are Active Against Planktonic and Biofilm Cells of Pseudomonas aeruginosa and Other Cystic Fibrosis-Associated Bacterial Pathogens

Frontiers in Microbiology
Arianna PompilioGiovanni Di Bonaventura

Abstract

A novel, electrochemically synthesized, silver nanoparticles (AgNPs) formulation was evaluated in vitro against Pseudomonas aeruginosa, Burkholderia cepacia, Stenotrophomonas maltophilia, and Staphylococcus aureus strains from cystic fibrosis (CF) patients. AgNPs were particularly active against P. aeruginosa and B. cepacia planktonic cells (median MIC: 1.06 and 2.12 μg/ml, respectively) by a rapid, bactericidal and concentration-dependent effect. AgNPs showed to be particularly effective against P. aeruginosa and S. aureus biofilm causing a viability reduction ranging from 50% (1×MIC) to >99.9% (4×MIC). Electron microscopy showed that AgNPs deconstruct extracellular matrix of P. aeruginosa biofilm, and accumulate at the cell surface causing cell death secondary to membrane damage. Compared to Tobramycin, AgNPs showed comparable, or even better, activity against planktonic and biofilm P. aeruginosa cells. AgNPs at concentrations effective against B. cepacia and P. aeruginosa were not toxic to G. mellonella larvae. Our silver-based formulation might be an alternative to antibiotics in CF patients. Further in vitro and in vivo studies are warranted to confirm this therapeutic potential.

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Citations

Oct 30, 2019·Expert Review of Anti-infective Therapy·Samantha Flores-TreviñoElvira Garza-González
Jun 22, 2019·Biomedical Microdevices·Alexandre Gomes RodriguesAna Olívia De Souza
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Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Cecilia VelinoMichele Iafisco
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May 4, 2021·Frontiers in Microbiology·Oliver McNeillyCindy Gunawan
May 28, 2021·Clinical Microbiology Reviews·Joanna S Brooke

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Methods Mentioned

BETA
transmission electron microscopy

Software Mentioned

ZetaPALS Particle Sizing
GraphPad
Prism

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