Electrochemiluminescent competitive immunoassay for zearalenone based on the use of a mimotope peptide, Ru(II)(bpy)3 -loaded NiFe2 O4 nanotubes and TiO2 mesocrystals

Mikrochimica Acta
Dandan FangYanyu Lin

Abstract

An ultrasensitive competitive-type electrochemiluminescence immunoassay for the mycotoxin zearalenone is described. The method is based on the use of (a) a mimotope peptide that was selected from a phage displayed peptide library and used to substitute ZEN for designing the competitive assay; (b) NiFe2O4 nanotubes with large specific surface area loaded with the ECL probe Ru(bpy)32+; and (c) poly(vinylpyrrolidone) (PVP)-assisted synthesis of TiO2 mesocrystals that acts as the sensing platform and support for antibody immobilization. Under the optimized conditions and at an ECL working potential of 1.1 V, a linear response is found for ZEN in the 0.1 to 1.0 × 10-5 ng·mL-1 concentration range with a detection limit as low as 3.3 fg·mL-1. Graphical abstract An ultrasensitive competitive-type electrochemiluminescence (ECL) immunosensor based on mimotope peptide was constructed for the detection of Zearalenone.

References

Apr 7, 2009·Analytica Chimica Acta·Hyang Sook ChunSergey A Eremin

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Citations

Apr 8, 2021·Analytical and Bioanalytical Chemistry·Lili ZhangHanjie Wang
Sep 30, 2021·Journal of Agricultural and Food Chemistry·Dan-Tong HuangZhen-Lin Xu

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