Electrocyte (Na(+),K(+))ATPase inhibition induced by zinc is reverted by dithiothreitol

The International Journal of Biochemistry & Cell Biology
M G L RibeiroA Hasson-Voloch

Abstract

The Mg(2+)-dependent (Na(+),K(+))ATPase maintains several cellular processes and is essential for cell excitability. In view of the importance of the enzyme activity, the interaction and binding affinities to substrates and metal ions have been studied. We determined the effect of Zinc ion (Zn(2+)) on the (Na(+),K(+))ATPase activity present in both conducting (non-innervated) and post-synaptic (innervated) membranes of electrocyte from Electrophorus electricus (L.). Zn(2+) is involved in many biological functions and is present in pre-synaptic nerve terminals. This metal, which has affinity for thiol groups, acted as a potent competitive inhibitor of (Na(+),K(+))ATPase of both membrane fractions, which were obtained by differential centrifugation of the E. electricus main electric organ homogenate. We tried to recover the enzyme activity using dithiothreitol, a reducing agent. Kinetic analysis showed that dithiothreitol acted as a non-essential non-competitive activator of (Na(+),K(+))ATPase from both membrane fractions and was able to revert the Zn(2+) inhibition at mM concentrations. In the presence of dithiothreitol, this metal behaved as a competitive inhibitor of (Na(+),K(+))ATPase in the non-innervated membrane fractions ...Continue Reading

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Citations

Mar 5, 2005·Nucleic Acids Research·Sreeparna Banerjee, Hernan Flores-Rozas
Jun 7, 2015·Current Opinion in Pharmacology·Brendan J Canning, David G S Farmer
Feb 18, 2004·Biochimica Et Biophysica Acta·J LoweA Hassón-Voloch
Feb 10, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Gülüzar Atli, Mustafa Canli
Mar 24, 2017·ZooKeys·Philippe V AlvesJosé L Luque
Mar 29, 2014·Pharmacological Reviews·P V DicpinigaitisC P Page

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