Electrode-based approach for monitoring in situ microbial activity during subsurface bioremediation

Environmental Science & Technology
Kenneth H WilliamsDerek R Lovley

Abstract

Current production by microorganisms colonizing subsurface electrodes and its relationship to substrate availability and microbial activity was evaluated in an aquifer undergoing bioremediation. Borehole graphite anodes were installed downgradient from a region of acetate injection designed to stimulate bioreduction of U(VI); cathodes consisted of graphite electrodes embedded at the ground surface. Significant increases in current density (< or =50 mA/m2) tracked delivery of acetate to the electrodes, dropping rapidly when acetate inputs were discontinued. An upgradient control electrode not exposed to acetate produced low, steady currents (< or =0.2 mA/m2). Elevated current was strongly correlated with uranium removal but minimal correlation existed with elevated Fe(II). Confocal laser scanning microscopy of electrodes revealed firmly attached biofilms, and analysis of 16S rRNA gene sequences indicated the electrode surfaces were dominated (67-80%) by Geobacter species. This is the first demonstration that electrodes can produce readily detectable currents despite long-range (6 m) separation of anode and cathode, and these results suggest that oxidation of acetate coupled to electron transfer to electrodes by Geobacter species...Continue Reading

References

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Citations

Jun 5, 2012·Bioelectrochemistry·Alessandro A Carmona-MartínezUwe Schröder
Jun 27, 2013·BMC Microbiology·Theodore M FlynnJorge W Santo Domingo
Jul 4, 2012·Annual Review of Microbiology·Derek R Lovley
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Feb 22, 2011·Current Opinion in Biotechnology·Derek R Lovley, Kelly P Nevin
Jan 22, 2011·Biotechnology Advances·Yu ZhangNico Boon
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Feb 1, 2011·Environmental Microbiology Reports·Derek R Lovley
Apr 16, 2011·Biotechnology and Bioengineering·Zhiqiang LiKelvin B Gregory
Sep 1, 2015·Frontiers in Microbiology·Sanja AracicAshley E Franks
Jun 7, 2014·Critical Reviews in Microbiology·Abhilasha Singh Mathuriya, J V Yakhmi
Jun 27, 2015·Bioresource Technology·Dawn E HolmesDerek R Lovley
Nov 20, 2012·Current Opinion in Biotechnology·Kenneth H WilliamsDerek R Lovley
Dec 9, 2014·Frontiers in Microbiology·Colin WardmanDerek R Lovley
Oct 7, 2018·Applied and Environmental Microbiology·Melany Ruiz-UrigüenPeter R Jaffé
Sep 4, 2019·Applied Microbiology and Biotechnology·Charles E TurickScott Greenway
Feb 11, 2019·Environmental Science and Pollution Research International·Panagiotis KirmizakisLeonid Kulakov
Oct 7, 2017·Microbial Biotechnology·Narcís PousSebastià Puig
Jul 30, 2021·The Science of the Total Environment·Wenbo YouMaosheng Zheng

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