Oct 1, 1975

Electrodecantation of serum proteins

Hoppe-Seyler's Zeitschrift für physiologische Chemie
T Kranz, F Lappe

Abstract

The sedimentation of albumin under the action of the electric and gravitational fields was determined as a function of time in discontinuous experiments in a rectangular cell, using serum with the albumin fraction stained blue. It was shown that even under the influence of strong electric fields, the upper boundary of the albumin layer fell no further than the mid-point of the cell. In continuous single-stage separation of gamma-globulin from other serum proteins, only about half the gamma-globulins can be obtained from the solution because it remains homogeneously distributed throughout the solution and is only free from albumin and other proteins in the upper half of the cell. In experiments with continuously operated triangular cells, the process was optimized to give gamma-globulin of 97.5% purity in a yield of 80%, at serum flow-through rates of up to 0.5 l/h in a block composed of 40 cells.

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Mentioned in this Paper

ALB
Serum Proteins
Electric Conductivity
Pseudoglobulins
Plasma Albumin
Hydrogen-Ion Concentration
Mathematics
Blood Protein Electrophoresis

About this Paper

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