Electroencephalographic evaluation of decapitation of the anaesthetized rat

Laboratory Animals
Kavitha KongaraCraig B Johnson

Abstract

The objective of this study was to evaluate the changes in electroencephalographic (EEG) power spectrum in response to decapitation of anaesthetized rats, in order to assess the nociception or otherwise of this procedure. Ten young adult male Sprague-Dawley rats were anaesthetized with halothane in oxygen and anaesthesia was maintained at a stable concentration of halothane between 1.20% and 1.25%. The rat's head and neck were placed through the opening of a small animal guillotine so that the blade of the guillotine was positioned over the atlanto-occipial joint of the rat's neck. The EEG was recorded in a five-electrode montage, bilaterally. After recording a 15 min baseline the rat was decapitated by swiftly pressing the guillotine blade and the EEG recording was continued until the signal was isoelectric on both channels. Changes in the median frequency (F50), 95% spectral edge frequency (F95) and total power of the EEG (Ptot) were used to investigate the effects of decapitation. During the first 15 s following decapitation, there were significant increases in the F50 and F95, and a decrease in the Ptot compared with baseline values. There was a clear window of time immediately following decapitation where changes in the EE...Continue Reading

References

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Jun 7, 1988·Brain Research·C H VanderwolfB Robertson
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Nov 3, 2010·Journal of the American Veterinary Medical Association·George Bates

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Citations

Sep 23, 2014·New Zealand Veterinary Journal·Cb JohnsonA D Fisher
Feb 10, 2011·PloS One·Clementina M van RijnAnton M L Coenen
Aug 27, 2016·Animals : an Open Access Journal From MDPI·Penny HawkinsHuw D R Golledge
Mar 25, 2015·Journal of Veterinary Internal Medicine·M AlemanJ E Madigan
Nov 7, 2019·Animals : an Open Access Journal From MDPI·Aline R SteinerThomas C Gent
Dec 15, 2019·Progress in Neurobiology·Adrien E SchrammStéphane Charpier

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Methods Mentioned

BETA
chip

Software Mentioned

Graph Pad
Spectral Analyser
Prism

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