PMID: 6411506Jul 1, 1983Paper

Electrofocusing fractionation and characterization of pituitary follicle-stimulating hormone from male and female rats

Molecular and Cellular Endocrinology
L M Foulds, D M Robertson

Abstract

Extracts of male and female rat pituitaries were fractionated by electrofocusing on sucrose density gradients and the pH distribution of FSH determined by an FSH in vitro bioassay, rFSH radioimmunoassay (RIA) and FSH radioreceptor assay (RRA) method. FSH for both sexes was located in the pH range 3-6. Significantly more activity, as measured by all three methods, was found in the male in the pH range 3.4-4.0 (P less than 0.01), while conversely significantly more activity was found in the female in the pH range 4.8-6.0 (P less than 0.05). Ratios of the levels of FSH assayed by the three methods showed significant (P less than 0.05) differences between sexes in both the original pituitary extracts and electrofocusing fractions in the pH region 4.4-6.0. The acidic fractions, when fractionated by gel filtration (Sephadex G100SF), were larger in apparent molecular size than the more alkaline fractions found in pituitary extracts from either sex. Binding of electrofocusing fractions from male pituitaries to concanavalin A showed significant (P less than 0.01) differences between fractions, suggesting that the various FSH fractions may differ in their carbohydrate structure. It is concluded that the differences in apparent size and c...Continue Reading

References

Sep 1, 1975·The Journal of Clinical Endocrinology and Metabolism·K W Cheng
Nov 1, 1971·The Journal of Clinical Endocrinology and Metabolism·D Tulchinsky, G E Abraham

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Citations

Jul 1, 1986·Molecular and Cellular Endocrinology·R I McLachlanD M de Kretser
Nov 25, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·A IwasawaM Kamiyoshi
May 1, 1988·General and Comparative Endocrinology·H Ando, S Ishii
Dec 1, 1991·Journal of Endocrinological Investigation·M Simoni, E Nieschlag
Feb 15, 2019·Frontiers in Endocrinology·Stella CampoSusana B Rulli
Nov 7, 2006·Molecular and Cellular Endocrinology·S CampoC Bergadá

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