Electrogenerated chemiluminescence detection of amino acids based on precolumn derivatization coupled with capillary electrophoresis separation.

Analytical Chemistry
Jianguo LiHuangxian Ju

Abstract

A novel method for highly sensitive detection of primary and secondary amino acids with selective derivatization using acetaldehyde as a new derivatization reagent was proposed by capillary electrophoresis (CE) coupled with electrogenerated chemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(II). The precolumn derivatization of these amino acids with acetaldehyde was performed in aqueous solution at room temperature for 1 h. Upon optimized derivatization, the ECL intensities and detection sensitivities of the amino acids were significantly enhanced by 20-70 times. Using four amino acids, arginine, proline, valine, and leucine, as model compounds, their derivatives could be completely separated by CE and sensitively detected by ECL within 22 min. The linear ranges were 0.5-100 microM for arginine and proline and 5-1000 microM for valine and leucine with the detection limits of 1 x 10(-7) (0.5 fmol, arginine), 8 x 10(-8) (0.4 fmol, proline), 1 x 10(-6) (5 fmol, valine), and 1.6 x 10(-6) M (8 fmol, leucine) at a signal-to-noise ratio of 3. The derivatization reactions and ECL process of amino acids were also proposed based on in situ Fourier transform infrared and ultraviolet spectrometric analyses.

References

Oct 21, 1983·Science·J W Jorgenson, K D Lukacs
Aug 1, 1983·Analytical Chemistry·I RubinsteinA J Bard
Feb 13, 2002·Analytical Chemistry·Samuel P Fowry, R Mark Wightman
Sep 28, 2002·Journal of Chromatography. a·Kazuhiko TsukagoshiRiichiro Nakajima
Dec 18, 2002·Journal of Pharmaceutical and Biomedical Analysis·Takeshi FukushimaKazuhiro Imai
Sep 16, 2003·Electrophoresis·Mei-Tsu ChiangChen-Wen Whang

❮ Previous
Next ❯

Citations

Dec 25, 2010·Analytical and Bioanalytical Chemistry·Longhua GuoGuonan Chen
May 21, 2013·Analytical and Bioanalytical Chemistry·Roberto MandrioliMaria Augusta Raggi
May 20, 2008·Analytical Chemistry·Vratislav KostalEdgar A Arriaga
Sep 16, 2010·Chemical Communications : Chem Comm·Yafeng WuSongqin Liu
Aug 10, 2011·Chemical Communications : Chem Comm·Gui-zheng ZouXiao-li Zhang
Nov 14, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ming Li, Sang Hak Lee
Oct 31, 2015·Biosensors & Bioelectronics·Mohtashim H ShamsiAaron R Wheeler
Sep 19, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Dongguang YinMinghong Wu
Sep 10, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Fakhr Eldin O SulimanSalama B Salama
Jun 12, 2012·Luminescence : the Journal of Biological and Chemical Luminescence·Huan YuTianyan You
Dec 16, 2011·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Jing Li, Erkang Wang
Dec 7, 2007·Electrophoresis·Véréna PoinsotFrançois Couderc
Sep 17, 2009·Luminescence : the Journal of Biological and Chemical Luminescence·Zhimin CaiXi Chen
Oct 21, 2009·Journal of Separation Science·Zhifeng FuWei Wei
Jun 22, 2010·Electrophoresis·Francisco J LaraAurelia Ibáñez Velasco
Apr 1, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Qiao-Hua WeiGuo-Nan Chen
Jan 1, 2011·Analytical Methods : Advancing Methods and Applications·Guo GuangmeiXi Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.