Electrogenerated chemiluminescence of luminol for oxidase-based fibre-optic biosensors

Luminescence : the Journal of Biological and Chemical Luminescence
Christophe A MarquetteLoïc J Blum

Abstract

The luminol electrochemiluminescence has been exploited for the development of several fibre-optic biosensors allowing the detection of hydrogen peroxide and of substrates of H(2)O(2)-producing oxidases. Electro-optical flow injection analysis of glucose, lactate, cholesterol and choline are thus described. To perform the experiments, a glassy carbon electrode was polarized at a fixed potential. Luminol was then electrochemically oxidized and could react in the presence of hydrogen peroxide to produce light. Several parameters had to be optimized to obtain reliable optical biosensors. An optimum applied potential of +425 mV between the glassy carbon electrode and the platinum pseudo-reference electrode was determined, allowing the best signal: noise ratio to be obtained. It was also necessary to optimize the experimental conditions for the immobilization of the different oxidases involved (preactivated membranes, chemically activated collagen membranes, photopolymerized matrix). For each biosensor developed, the optimum reaction conditions have been studied: buffer composition, pH, temperature, flow rate and luminol concentration. Under optimal conditions, the detection limits (S/N = 3) were 30 pmol, 60 pmol, 0.6 nmol and 10 pm...Continue Reading

References

Aug 1, 1974·Biotechnology and Bioengineering·P R CouletD C Gautheron

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Citations

May 23, 2006·Analytical and Bioanalytical Chemistry·Christophe A Marquette, Loïc J Blum
Oct 3, 2007·Analytical and Bioanalytical Chemistry·Christophe A Marquette, Loïc J Blum
Apr 25, 2009·Analytical and Bioanalytical Chemistry·Audrey SassolasBéatrice D Leca-Bouvier
Jul 22, 2016·Biosensors·Vikramshankar KamakotiShalini Prasad
Jun 19, 2014·Analytical and Bioanalytical Chemistry·Béatrice D Leca-BouvierLoïc J Blum
Oct 25, 2002·Teratology·Gautam S GhatnekarIda W Smoak
Jul 5, 2012·Advanced Materials·Jian Yao ZhengJiannian Yao
Feb 23, 2020·Current Medicinal Chemistry·Minmin ChangJunli Ren
Mar 15, 2003·Biochimica Et Biophysica Acta·Giovanni Gadda
Jan 31, 2008·Chemical Reviews·Sergey M Borisov, Otto S Wolfbeis

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