Electrogenerated chemiluminescence of tris(2,2' bipyridine)ruthenium(II) using common biological buffers as co-reactant, pH buffer and supporting electrolyte

The Analyst
Noah KebedeConor F Hogan

Abstract

A series of aliphatic tertiary amines (HEPES, POPSO, EPPS and BIS-TRIS) commonly used to buffer the pH in biological experiments, were examined as alternative, non-toxic co-reactants for the electrogenerated chemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(ii) ([Ru(bpy)3](2+)). These were found to be very attractive as "multi-tasking" reagents, serving not only as co-reactants, but also fulfiling the roles of pH buffer and supporting electrolyte within an aqueous environment; thus significantly simplifying the overall ECL analysis. Sub-nanomolar detection limits were obtained for [Ru(bpy)3](2+) in the presence of BIS-TRIS, making this species an valuable option for co-reactant ECL-based bioanalytical applications.

References

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Citations

Oct 13, 2016·Physical Chemistry Chemical Physics : PCCP·Haidong LiNeso Sojic
Mar 5, 2016·Analytical and Bioanalytical Chemistry·Shimeles Addisu KitteGuobao Xu
Aug 16, 2016·Analytical and Bioanalytical Chemistry·Aila Jimenez-RuizConcepcion Torres-Marquez
Aug 14, 2020·Physical Chemistry Chemical Physics : PCCP·David IbáñezPablo Fanjul-Bolado
Oct 25, 2017·Journal of the American Chemical Society·Giovanni ValentiNeso Sojic
Nov 30, 2021·Analytical Chemistry·Minghui FengNeso Sojic

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