Jan 25, 1978

Electrogenic behavior of synaptic vesicles from Torpedo californica

The Journal of Biological Chemistry
R S Carpenter, S M Parsons

Abstract

Electrical potential changes in pure synaptic vesicles from Torpedo californica were monitored with the fluorescent dye 3,3'-dipropylthiadicarbocyanine iodide. Vesicles resuspended in variable external sodium ion in the presence of gramicidin established sodium ion membrane diffusion potentials. Vesicles resuspended in choline or acetylcholine chloride became hyperpolarized upon addition of gramicidin. Hyperpolarization was subsequently partially reversed spontaneously by choline or acetylcholine influx, which was confirmed by gel filtration, to yield a new, less negative, stable membrane potential. Thus, acetylcholine and choline are taken up electrogenically by synaptic vesicles.

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Mentioned in this Paper

Choline
Resting Potentials
Synaptic Vesicles
Gramicidin NF
Vesicle
Fluorescent stain
Gel Chromatography
Sodium Cation
Sodium
Torpedo californica

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