PMID: 8956504Jan 1, 1996Paper

Electrogenic cation transport across leech caecal epithelium

Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
H MildeW M Weber

Abstract

Electrogenic cation transport across the caecal epithelium of the leech Hirudo medicinalis was investigated using modified Ussing chambers. Transepithelial resistance (RT) and potential difference (VT) were 61.0 +/- 3.5 omega.cm2 and -1.1 +/- 0.2 mV (n = 149), respectively, indicating that leech caecal epithelium is a "leaky" epithelium. Under control conditions short circuit current (ISC) and transepithelial Na+ transport rate (INa) averaged at 22.1 +/- 1.5 microA.cm-2 and 49.7 +/- 2.6 microA.cm-2, respectively. Mucosal application of amiloride (100 mumol.l-1) or benzamil (50 mumol.l-1) influenced neither ISC nor INa. The transport system in the apical membrane showed no pronounced cation selectivity and a linear dependence on mucosal Na+ concentration. Removal of mucosal Ca2+ increased ISC by about 50% due to an increase of transepithelial Na+ transport. Trivalent cations (La3+ and Tb3+, 1 mmol.l-1 both) added to the mucosal Ringer solution reduced INa by more than 40%. Serosal ouabain (1 mmol.l-1) almost halved ISC and INa while 0.1% (= 5.4 mmol.l-1) DNP decreased INa to 11.8 +/- 5.1% of initial values. Serosal addition of cAMP increased both ISC and INa whereas the neurotransmitters. FMRFamide, acetylcholine, GABA, L-dopa, ...Continue Reading

References

Dec 1, 1992·Comparative Biochemistry and Physiology. Comparative Physiology·D Cremaschi, C Porta
Apr 1, 1991·Pflügers Archiv : European journal of physiology·W Van DriesscheJ Simaels
Jan 1, 1991·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·M HeinzW Clauss
Sep 1, 1991·Peptides·B D EvansR L Calabrese
Jan 1, 1990·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·R KrattenmacherW Clauss
Oct 1, 1985·Pflügers Archiv : European journal of physiology·W Van Driessche, W Zeiske
Apr 1, 1989·Physiological Reviews·L Reuss
Jan 1, 1972·The Journal of Membrane Biology·E Frömter
Jan 1, 1984·Annual Review of Physiology·B Lindemann
Jan 1, 1994·The Journal of General Physiology·T A HemingL Reuss
Jan 1, 1978·The Review of Scientific Instruments·W Van Driessche, B Lindemann

❮ Previous
Next ❯

Citations

Jul 4, 1997·Molecular and Cellular Endocrinology·M Salzet, G Stefano
Mar 3, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Katja SobczakWolf-Michael Weber
Nov 8, 2002·Biochimica Et Biophysica Acta·Mikael SchnizlerWolfgang Clauss

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.