Electrogenic K+ transport by the Na(+)-K+ pump in rat cardiac ventricular myocytes

The Journal of Physiology
R Daniel Peluffo, Joshua R Berlin

Abstract

1. The involvement of electrogenic reaction steps in K+ transport by the Na+, K(+)-ATPase was determined in rat cardiac ventricular myocytes using whole-cell patch clamp techniques. 2. Under K(+)-K+ exchange conditions and in the presence of extracellular K+ or Tl+ at concentrations that stimulated submaximal levels of steady-state Na+,K(+)-ATPase activity, ouabain-sensitive transient currents were observed during ('on') and after ('off') step changes in membrane potential (Vm). 3. The quantity of charge moved during the transient currents depended, in a saturable manner, on the magnitude of the voltage step. Maximal ouabain-sensitive 'on' and 'off' charges were calculated to be 9.6 +/- 0.9 and 9.1 +/- 0.4 fC pF-1 (n = 4), respectively, with an effective valeney of 0.48 +/- 0.07 (n = 7). 4. Kinetics of the transient currents were independent of Vm and Tl+o at positive potentials, but became more rapid at increasingly negative Vm values in an ion concentration-dependent fashion. 5. Those data demonstrate that electrogenic steps participate in K+ transport by the Na+,K(+)-ATPase and that the electrogenic step is extracellular ion binding. 6. The temperature- and Vm-dependent properties of transient charge movements were compared ...Continue Reading

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Citations

Feb 4, 2006·The Journal of Membrane Biology·J A Hernández, J Fischbarg
Sep 24, 2013·Journal of Bioenergetics and Biomembranes·Sergey M KorotkovArtemy V Novozhilov
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Siddhartha YaragatupalliPablo Artigas
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