Electroimmunoassay of sex hormone binding globulin. Enhanced sensitivity by autoradiography using A-ring 125I-17-beta-oestradiol

Scandinavian Journal of Clinical and Laboratory Investigation
P FernlundG Rannevik

Abstract

Four different 125I-iodinated steroids were tested for their binding to human sex hormone binding globulin (SHBG) using an electrophoretic technique. 17-beta-oestradiol iodinated in its A-ring was found to bind with high affinity to SHBG. This radioactive steroid was used to increase the sensitivity of the electroimmunoassay of SHBG by adding the steroid to the samples before electroimmunoassay. The radioactive steroid incorporated into the immunoprecipitates could be observed by autoradiography. The sensitivity of the assay, which employed a rabbit antiserum against purified human SHBG and was standardized with pure SHBG, was about 0.2 mg/l. The precision of the method, calculated as the coefficient of variation within and between assays, was 2.4% and 2.6%, respectively, for values within the normal range. The mean SHBG concentration in healthy regularly menstruating women (n = 50) was 3.50 +/- 0.74 (SD) mg/l when measured in plasma, and 3.78 +/- 0.80 mg/l when measured in serum. The corresponding mean concentrations in healthy men (n = 28) were 2.26 +/- 0.45 mg/l in plasma and 2.44 +/- 0.49 mg/l in serum. The modified electroimmunoassay described in this paper is a simple modification, which increases the sensitivity sufficie...Continue Reading

References

May 1, 1976·British Journal of Obstetrics and Gynaecology·C M TowlerH Bohn
Mar 11, 1975·Biochemistry·K E Mickelson, P H Pétra
Oct 1, 1979·The Journal of Clinical Endocrinology and Metabolism·M Malmquist, J I Thorell
Nov 1, 1979·The Journal of Clinical Endocrinology and Metabolism·C B Laurell, G Rannevik
Aug 1, 1978·Journal of Steroid Biochemistry·D R AbramovichH Bohn
May 1, 1979·Journal of Steroid Biochemistry·M J Iqbal, M W Johnson
Jul 1, 1979·Journal of Steroid Biochemistry·C Mercier-BodardE E Baulieu
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·B G Johansson
Jan 1, 1974·Clinical Endocrinology·D C Anderson
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R B Hochberg, W Rosner
Aug 1, 1980·The Journal of Clinical Endocrinology and Metabolism·J F DunnB C Nisula
Apr 1, 1982·The Journal of Clinical Endocrinology and Metabolism·M S KhanW Rosner

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Citations

Mar 1, 1989·Journal of Steroid Biochemistry·S ErikssonG Rannevik
Jun 1, 1990·Journal of Steroid Biochemistry·P Fernlund, S Gershagen
Sep 1, 1985·Scandinavian Journal of Clinical and Laboratory Investigation·P FernlundG Rannevik
Feb 1, 1985·Scandinavian Journal of Clinical and Laboratory Investigation·G LindstedtR Vihko

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