Electroinsertion and activation of the C-terminal domain of colicin A, a voltage gated bacterial toxin, into mammalian cell membranes

Molecular Membrane Biology
S RaffyJ Teissié

Abstract

The C-terminal fragment of colicin, a protein that is highly soluble in aqueous solution, is spontaneously and irreversibly inserted into the membranes of mammalian cells, which are locally permeabilized by a transmembrane voltage increase. Insertion is detected by immunodetection. This is obtained by mixing the protein with electropermeabilized cells. The same result is observed by pulsing the colicin/cell mixture. Electroinsertion is therefore obtained for the first time with a multi-fragment spanning protein. The cell viability is not affected beyond the effect of electropermeabilization. A train of low voltage repetitive transmembrane modulation, which cannot trigger membrane permeabilization, is applied a day after the electroinsertion. This induces no effect on unmodified cells but triggers the lysis of cells in which colicin has been inserted by the first electropulsation. The low-level electrical treatment is high enough to trigger the voltage gated opening of colicin and to induce the associated toxicity. A transmembrane configuration of colicin is therefore obtained by electroinsertion. The toxic effect of their voltage gating is only obtained when a critical number of voltage gated channels are activated.

References

Jun 1, 1979·European Journal of Biochemistry·D Cavard, C J Lazdunski
Apr 5, 1992·Journal of Molecular Biology·M W ParkerD Tsernoglou
Oct 1, 1991·Biophysical Journal·V A Klenchin Chizmadzhev YuA
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M ZeiraC Nicolau
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J P BourdineaudL Letellier
Apr 1, 1990·Trends in Biochemical Sciences·M W ParkerF Pattus
Feb 28, 1989·Biochemical and Biophysical Research Communications·Y MouneimneC Nicolau
Apr 27, 1987·Biochimica Et Biophysica Acta·M K Jain, D Zakim
Oct 15, 1986·Biochemical and Biophysical Research Communications·J Teissie, M P Rols
Jun 27, 1985·Biochimica Et Biophysica Acta·K Schwister, B Deuticke
Jan 1, 1982·Annual Review of Microbiology·J Konisky
Feb 6, 1981·Biochimica Et Biophysica Acta·G Stulen
Mar 17, 1981·Biochemistry·J Teissie, T Y Tsong
Mar 21, 1983·Biochimica Et Biophysica Acta·W A CramerY Uratani
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J TeissieJ Wehrle
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·W A CramerC V Stauffacher
Nov 10, 1995·The Journal of Biological Chemistry·K el OuagariH Benoist
Sep 5, 1993·Biochimica Et Biophysica Acta·K el OuagariJ Teissié
Nov 1, 1995·Biochemical Society Transactions·A Watts
Jan 30, 1997·Nature·M S Sansom
May 1, 1997·The Journal of Membrane Biology·P K KienkerK S Jakes
Jan 7, 2000·Journal of Molecular Biology·M LindebergW A Cramer
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stanislav D ZakharovWilliam A Cramer
Nov 1, 2002·Biochimica Et Biophysica Acta·Stanislav D Zakharov, William A Cramer
Apr 25, 2003·European Biophysics Journal : EBJ·Blaz ValicDamijan Miklavcic
Nov 1, 1992·Trends in Cell Biology·F G van der GootF Pattus

❮ Previous
Next ❯

Citations

Oct 8, 2014·The Journal of Membrane Biology·Samo Mahnič-KalamizaDamijan Miklavčič
Oct 19, 2005·Bioelectrochemistry·T R GowrishankarJames C Weaver

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.