PMID: 3754390Apr 1, 1986Paper

Electrolyte permeabilities of pancreatic zymogen granules: implications for pancreatic secretion

The American Journal of Physiology
R C De Lisle, U Hopfer

Abstract

Zymogen granules from rat pancreas were prepared on a 40% Percoll gradient at free calcium levels less than 0.2 microM. We have previously shown [Am. J. Physiol. 246 (Gastrointest. Liver Physiol. 9)] that zymogen granules prepared by this method are stable in vitro for more than 1 h in "physiological buffers." The electrolyte permeabilities of the zymogen granule membrane were investigated to determine the basis for this stability. Ionic permeabilities were estimated from rates of osmotic lysis and measured as decrease in optical density (OD) of granule suspensions. OD correlated linearly with lysis, as indicated by release of amylase, except for the highest and lowest 10% of the OD of intact granules. Lysis of freshly isolated granules was slow in Na+ or K+ salt solutions (e.g., t1/2 approximately 3 h for Cl-) but was accelerated 5- to 50-fold when cation ionophores were present simultaneously. This behavior indicates that zymogen granules have low endogenous permeabilities to the cations Na+ and K+, but are highly permeable to a variety of anions. Both anion conductance and anion-exchange pathways were found. The relative selectivity of the anion conductance pathway was SCN- greater than Br- approximately NO-3 greater than SO...Continue Reading

Citations

Aug 15, 2002·American Journal of Physiology. Cell Physiology·Frank Thévenod
Jan 16, 2008·Journal of Cellular and Molecular Medicine·H SugiyaS Hashimoto
Dec 13, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mitsuyoshi YamamotoGary M Green
Dec 31, 2002·Biochemical and Biophysical Research Communications·Frank ThévenodCatherine M Fuller

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