PMID: 2500569Jan 1, 1989Paper

Electromechanical effects of leukotriene D4 on ferret tracheal muscle and its muscarinic responsiveness

Lung
H K Lee, C G Murlas

Abstract

We investigated the possible electrophysiological processes by which leukotriene D4 (LTD4) affects airway smooth muscle and its responsiveness to acetylcholine (ACh). For study in vitro, preparations of ferret tracheal muscle (dissected free of overlying mucosal and submucosal layers) were used. These preparations were arranged so that force transducers and glass intracellular microelectrodes (having tip resistances of 35-60 megohm) could be used to measure isometric force generation and cell membrane potential (Em) simultaneously from muscle stimulated by LTD4. At rest, the muscle was electrically and mechanically quiescent and had an Em of -59 +/- 0.2 mV (mean +/- SEM). We found that ferret tracheal muscle cells were relatively sensitive to LTD4, and that both the resulting depolarization (beginning at 10(-10) M LTD4) and force generation (produced by higher concentrations) progressed in a concentration-dependent manner. Depolarization by 10(-9) M LTD4 elicited electrical oscillations. These oscillations were accompanied by phasic contractile activity at 5 x 10(-9) M LTD4. Verapamil abolished these oscillations and diminished force substantially. We also found that ACh depolarized and contracted the muscle in a concentration-...Continue Reading

References

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Citations

Oct 2, 1990·European Journal of Pharmacology·H K LeeN Sperelakis

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