Electromembrane extraction of gonadotropin-releasing hormone agonists from plasma and wastewater samples

Electrophoresis
Saeed NojavanSaeid Yaripour

Abstract

In the present study, for the first time electromembrane extraction followed by high performance liquid chromatography coupled with ultraviolet detection was optimized and validated for quantification of four gonadotropin-releasing hormone agonist anticancer peptides (alarelin, leuprolide, buserelin and triptorelin) in biological and aqueous samples. The parameters influencing electromigration were investigated and optimized. The membrane consists 95% of 1-octanol and 5% di-(2-ethylhexyl)-phosphate immobilized in the pores of a hollow fiber. A 20 V electrical field was applied to make the analytes migrate from sample solution with pH 7.0, through the supported liquid membrane into an acidic acceptor solution with pH 1.0 which was located inside the lumen of hollow fiber. Extraction recoveries in the range of 49 and 71% within 15 min extraction time were obtained in different biological matrices which resulted in preconcentration factors in the range of 82-118 and satisfactory repeatability (7.1 < RSD% < 19.8). The method offers good linearity (2.0-1000 ng/mL) with estimation of regression coefficient higher than 0.998. The procedure allows very low detection and quantitation limits of 0.2 and 0.6 ng/mL, respectively. Finally, i...Continue Reading

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Citations

Feb 6, 2021·Bioanalysis·Linda Vårdal EieStig Pedersen-Bjergaard
Feb 4, 2021·Journal of Chromatography. a·Frederik André Hansen, Stig Pedersen-Bjergaard
Apr 29, 2021·Journal of Separation Science·Frederik André HansenStig Pedersen-Bjergaard

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