Electromembrane extraction of substances with weakly basic properties: a fundamental study with benzodiazepines

Bioanalysis
Linda VårdalStig Pedersen-Bjergaard

Abstract

Electromembrane extraction (EME) of weakly basic benzodiazepines was investigated (-1.47 < pKa < 5.01). 96-well EME was performed with strongly acidic conditions in the acceptor solution using 250-mM trifluoroacetic acid to maximize ionization. Recoveries more than 80% were obtained for analytes with pKa > 2, whereas EME was less efficient for substances with pKa < 2. The latter was trapped in the supported liquid membrane due to less acidic pH conditions in the acceptor solution close to the supported liquid membrane. EME followed by UHPLC-MS/MS analysis was evaluated from human plasma, and the results were in compliance with EMA guidelines. Both electrokinetic migration and passive diffusion contributed to mass transfer when performing EME of weakly basic analytes.

References

Feb 1, 2006·Journal of Chromatography. a·Stig Pedersen-Bjergaard, Knut Einar Rasmussen
Apr 1, 2006·Journal of Chromatography. a·Mohammad RezaeeSana Berijani
Nov 28, 2006·Journal of Chromatography. a·Marte BalchenStig Pedersen-Bjergaard
May 16, 2008·Journal of Chromatography. a·Marte BalchenStig Pedersen-Bjergaard
Oct 31, 2008·Journal of Chromatography. a·Chanbasha BasheerHian Kee Lee
Jan 8, 2011·Journal of Pharmaceutical and Biomedical Analysis·Mahboube EskandariShahram Seidi
Feb 24, 2011·Journal of Separation Science·Shahram SeidiMorteza Moradi
Apr 16, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maryam RezazadehShahram Seidi
Oct 12, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nickolaj Jacob PetersenAstrid Gjelstad
Jun 19, 2012·Journal of Chromatography. a·Noelia Cabaleiro DomínguezStig Pedersen-Bjergaard
Oct 16, 2015·Electrophoresis·Saied Saeed Hosseiny DavaraniSaeed Nojavan
May 16, 2017·Analytical Chemistry·Nicolas DrouinJulie Schappler
Aug 28, 2017·Analytica Chimica Acta·Magnus Saed RestanStig Pedersen-Bjergaard

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Citations

Dec 20, 2018·Analytical and Bioanalytical Chemistry·Stig Pedersen-Bjergaard
Jan 2, 2021·Forensic Science International : Synergy·Wing-Sum ChanBobbie Kwok-Keung Cheung
Dec 11, 2020·Talanta·Hana Bavlovič PiskáčkováStig Pedersen-Bjergaard

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