PMID: 9435085Jan 22, 1998Paper

Electron and fluorescence microscopy of extracellular glucan and aryl-alcohol oxidase during wheat-straw degradation by Pleurotus eryngii

Applied and Environmental Microbiology
J M BarrasaA T Martinez

Abstract

The ligninolytic fungus Pleurotus eryngii grown in liquid medium secreted extracellular polysaccharide (87% glucose) and the H2O2-producing enzyme aryl-alcohol oxidase (AAO). The production of both was stimulated by wheat-straw. Polyclonal antibodies against purified AAO were obtained, and a complex of glucanase and colloidal gold was prepared. With these tools, the localization of AAO and extracellular glucan in mycelium from liquid medium and straw degraded under solid-state fermentation conditions was investigated by transmission electron microscopy (TEM) and fluorescence microscopy. These studies revealed that P. eryngii produces a hyphal sheath consisting of a thin glucan layer. This sheath appeared to be involved in both mycelial adhesion to the straw cell wall during degradation and AAO immobilization on hyphal surfaces, with the latter evidenced by double labelling. AAO distribution during differential degradation of straw tissues was observed by immunofluorescence microscopy. Finally, TEM immunogold studies confirmed that AAO penetrates the plant cell wall during P. eryngii degradation of wheat straw.

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Citations

Jun 12, 2009·Critical Reviews in Biotechnology·Mirjana StajićSonja Duletić-Lausević
Jul 31, 2007·Applied and Environmental Microbiology·Geoffrey DanielPetr Halada
Jan 18, 2012·Applied Microbiology and Biotechnology·Aitor Hernández-OrtegaAngel T Martínez
Nov 13, 2008·Critical Reviews in Microbiology·Chandralata RaghukumarAshutosh Kumar Verma
Sep 25, 2018·Engineering in Life Sciences·Doris JarosHarald Rohm
Apr 9, 2017·World Journal of Microbiology & Biotechnology·Marianne Daou, Craig B Faulds

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