Electron and Proton Flux for Carbon Dioxide Reduction in Methanosarcina barkeri During Direct Interspecies Electron Transfer

Frontiers in Microbiology
Dawn E HolmesDerek R Lovley

Abstract

Direct interspecies electron transfer (DIET) is important in diverse methanogenic environments, but how methanogens participate in DIET is poorly understood. Therefore, the transcriptome of Methanosarcina barkeri grown via DIET in co-culture with Geobacter metallireducens was compared with its transcriptome when grown via H2 interspecies transfer (HIT) with Pelobacter carbinolicus. Notably, transcripts for the F420H2 dehydrogenase, Fpo, and the heterodisulfide reductase, HdrABC, were more abundant during growth on DIET. A model for CO2 reduction was developed from these results in which electrons delivered to methanophenazine in the cell membrane are transferred to Fpo. The external proton gradient necessary to drive the otherwise thermodynamically unfavorable reverse electron transport for Fpo-catalyzed F420 reduction is derived from protons released from G. metallireducens metabolism. Reduced F420 is a direct electron donor in the carbon dioxide reduction pathway and also serves as the electron donor for the proposed HdrABC-catalyzed electron bifurcation reaction in which reduced ferredoxin (also required for carbon dioxide reduction) is generated with simultaneous reduction of CoM-S-S-CoB. Expression of genes for putative re...Continue Reading

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Citations

Jan 1, 2020·Biotechnology for Biofuels·Pinjing HeFan Lü
May 12, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Bing GuoYang Liu
Aug 25, 2019·The ISME Journal·Paola Andrea PalaciosAmelia-Elena Rotaru
Sep 16, 2020·Journal of Industrial Microbiology & Biotechnology·Dinesh GuptaArpita Bose
Nov 17, 2019·Bioresource Technology·Wangwang YanYan Zhou
Feb 5, 2021·Current Opinion in Biotechnology·Amelia-Elena RotaruFlorin Musat
Jul 18, 2020·Environmental Research·Qian ChenDawn E Holmes
Mar 26, 2021·Bioresource Technology·Qiuxian QiYen Wah Tong
Apr 9, 2021·Frontiers in Microbiology·Kailin Gao, Yahai Lu
May 2, 2021·Applied and Environmental Microbiology·Jinjie ZhouDerek R Lovley

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Datasets Mentioned

BETA
PRJNA501858

Methods Mentioned

BETA
RNA-Seq
Hi-Seq
Illumina sequencing

Software Mentioned

FASTQC
Pfam
SortMeRNA
Integrated Genomes ( IMG )
HMMTOP
TMpred
ArrayStar
trimmomatic
PSORTb
TMHMM

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