Electron and Proton Flux for Carbon Dioxide Reduction in Methanosarcina barkeri During Direct Interspecies Electron Transfer

Frontiers in Microbiology
Dawn E HolmesDerek R Lovley


Direct interspecies electron transfer (DIET) is important in diverse methanogenic environments, but how methanogens participate in DIET is poorly understood. Therefore, the transcriptome of Methanosarcina barkeri grown via DIET in co-culture with Geobacter metallireducens was compared with its transcriptome when grown via H2 interspecies transfer (HIT) with Pelobacter carbinolicus. Notably, transcripts for the F420H2 dehydrogenase, Fpo, and the heterodisulfide reductase, HdrABC, were more abundant during growth on DIET. A model for CO2 reduction was developed from these results in which electrons delivered to methanophenazine in the cell membrane are transferred to Fpo. The external proton gradient necessary to drive the otherwise thermodynamically unfavorable reverse electron transport for Fpo-catalyzed F420 reduction is derived from protons released from G. metallireducens metabolism. Reduced F420 is a direct electron donor in the carbon dioxide reduction pathway and also serves as the electron donor for the proposed HdrABC-catalyzed electron bifurcation reaction in which reduced ferredoxin (also required for carbon dioxide reduction) is generated with simultaneous reduction of CoM-S-S-CoB. Expression of genes for putative re...Continue Reading


Oct 9, 2001·Bioinformatics·G E Tusnády, I Simon
Apr 3, 2003·Chembiochem : a European Journal of Chemical Biology·Mario TietzeUwe Beifuss
Aug 4, 2007·Archives of Microbiology·Hope A Johnson, Bradley M Tebo
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jul 1, 2008·Nature Reviews. Microbiology·Rudolf K ThauerReiner Hedderich
Jan 26, 2010·Biochemical and Biophysical Research Communications·Takuya OgawaHisashi Hemmi
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anne-Kristin KasterRudolf K Thauer
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Jul 28, 2012·Frontiers in Microbiology·Petra R A Kohler, William W Metcalf
Oct 17, 2012·Bioinformatics·Evguenia KopylovaHélène Touzet
Nov 6, 2012·Nucleic Acids Research·Sarah W BurgeAlex Bateman
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Jan 30, 2013·Biochemical Society Transactions·Katharina Schlegel, Volker Müller
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Illumina sequencing

Software Mentioned

Integrated Genomes ( IMG )

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