Electron Beam Immobilization of Novel Antimicrobial, Short Peptide Motifs Leads to Membrane Surfaces with Promising Antibacterial Properties

Journal of Functional Biomaterials
André ReinhardtInes Neundorf

Abstract

In this study, the efficacy of electron beam irradiation versus chemical coupling for yielding polyethersulfone (PES) membranes with antibacterial properties was investigated. For the surface coating, a recently discovered lead compound, IL-KKA, comprising a short peptide sequence functionalized with imidazolium groups, was used. For better integration within the membrane, several novel variants of IL-KKA were generated. Membrane immobilization was achieved using different doses of electron beam irradiation and NHS/EDC chemical coupling. Physicochemical characterization of the coated membranes was performed by water contact angle measurements, X-ray photoelectron spectroscopy, and scanning electron microscopy. Our results show that electron beam irradiation is as effective and gentle as chemical coupling using the NHS/EDC method. Moreover, it was demonstrated that the obtained membranes exhibit promising antibacterial activity againstB. subtilis. In summary, the technique presented herein might be promising as a template for developing future anti-biofilm devices.

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Citations

Nov 6, 2018·Scientific Reports·Mareike Horn, Ines Neundorf
Nov 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jorge L Díaz-GómezSilverio García-Lara

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Methods Mentioned

BETA
environmental stresses
X-ray
scanning electron microscopy

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MultiSynTech

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