Electron bifurcation involved in the energy metabolism of the acetogenic bacterium Moorella thermoacetica growing on glucose or H2 plus CO2.

Journal of Bacteriology
Haiyan HuangRudolf K Thauer

Abstract

Moorella thermoacetica ferments glucose to three acetic acids. In the oxidative part of the fermentation, the hexose is converted to 2 acetic acids and 2 CO(2) molecules with the formation of 2 NADH and 2 reduced ferredoxin (Fd(red)(2-)) molecules. In the reductive part, 2 CO(2) molecules are reduced to acetic acid, consuming the 8 reducing equivalents generated in the oxidative part. An open question is how the two parts are electronically connected, since two of the four oxidoreductases involved in acetogenesis from CO(2) are NADP specific rather than NAD specific. We report here that the 2 NADPH molecules required for CO(2) reduction to acetic acid are generated by the reduction of 2 NADP(+) molecules with 1 NADH and 1 Fd(red)(2-) catalyzed by the electron-bifurcating NADH-dependent reduced ferredoxin:NADP(+) oxidoreductase (NfnAB). The cytoplasmic iron-sulfur flavoprotein was heterologously produced in Escherichia coli, purified, and characterized. The purified enzyme was composed of 30-kDa (NfnA) and 50-kDa (NfnB) subunits in a 1-to-1 stoichiometry. NfnA harbors a [2Fe2S] cluster and flavin adenine dinucleotide (FAD), and NfnB harbors two [4Fe4S] clusters and FAD. M. thermoacetica contains a second electron-bifurcating enz...Continue Reading

References

Oct 21, 1976·Journal of Theoretical Biology·P Mitchell
Nov 1, 1978·Journal of Bacteriology·G B Diekert, R K Thauer
Jan 1, 1978·Methods in Enzymology·L G Ljungdahl, J R Andreesen
Mar 1, 1977·Bacteriological Reviews·R K ThauerK Decker
Jun 15, 1975·FEBS Letters·L G Ljungdahl, J R Andreesen
Jan 1, 1986·Annual Review of Microbiology·L G Ljungdahl
May 30, 1973·Biochimica Et Biophysica Acta·K JungermannK Decker
Aug 1, 1984·Journal of Bacteriology·L L Lundie, H L Drake
Jun 6, 2003·FEBS Letters·Carola HunteBernard L Trumpower
Jan 6, 2005·Research in Microbiology·Harold L Drake, Steven L Daniel
Jun 1, 1942·Journal of Bacteriology·F E FontaineG J Ritter
Jul 4, 2007·Biochemistry·Anssi M MalinenReijo Lahti
Nov 28, 2007·Journal of Bacteriology·Gloria HerrmannWolfgang Buckel
Jul 1, 2008·Nature Reviews. Microbiology·Rudolf K ThauerReiner Hedderich
Jul 18, 2008·Environmental Microbiology·Elizabeth PierceStephen W Ragsdale
Sep 20, 2008·Biochimica Et Biophysica Acta·Stephen W Ragsdale, Elizabeth Pierce
Jun 30, 2009·Nature Chemical Biology·Bryson D BennettJoshua D Rabinowitz
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Michael KöpkePeter Dürre

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Citations

Jul 20, 2012·The Journal of Biological Chemistry·Kai Schuchmann, Volker Müller
Jun 12, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Filipa L SousaWilliam F Martin
Apr 5, 2014·Astrobiology·Michael J RussellIsik Kanik
Mar 13, 2013·The Journal of Biological Chemistry·Johannes BertschVolker Müller
Feb 4, 2016·Biotechnology Reports·Ciarán L KellyFrank Sargent
Jun 29, 2014·Biochimica Et Biophysica Acta·Petra WormAlfons J M Stams
Dec 5, 2015·Frontiers in Microbiology·Martijn DienderDiana Z Sousa
Apr 2, 2016·Bioresource Technology·Shantonu RoyDeepak Pant
Aug 19, 2015·Frontiers in Microbiology·Sebastiaan K SpaansServé W M Kengen
Dec 6, 2012·FEMS Microbiology Reviews·Boran KartalJan T Keltjens
Oct 30, 2012·Biochimica Et Biophysica Acta·Arren Bar-Even
May 28, 2014·Current Opinion in Biotechnology·Haythem LatifKarsten Zengler
Oct 16, 2012·Biochimica Et Biophysica Acta·Elbert Branscomb, Michael J Russell
May 21, 2015·PloS One·Rebeca González-CabaleiroJorge Rodríguez
Jun 1, 2016·Biochimica Et Biophysica Acta·Saroj PoudelEric S Boyd
Jun 25, 2016·Extremophiles : Life Under Extreme Conditions·Ronja BreitkopfRalf-Jörg Fischer
Oct 22, 2015·Annual Review of Microbiology·Rudolf Kurt Thauer
Oct 14, 2016·Frontiers in Microbiology·Jongoh ShinByung-Kwan Cho
Sep 15, 2016·Extremophiles : Life Under Extreme Conditions·Mirko Basen, Volker Müller
Apr 11, 2017·Nature Chemical Biology·Carolyn E LubnerJohn W Peters
Nov 5, 2015·Scientific Reports·Anupam Chowdhury, Costas D Maranas
Nov 11, 2014·Nature Reviews. Microbiology·Kai Schuchmann, Volker Müller
May 23, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·M Ahsanul IslamGregory Stephanopoulos
Jun 21, 2018·Annual Review of Microbiology·Volker MüllerMirko Basen
Jul 9, 2014·Journal of Bacteriology·Johanna MockRudolf K Thauer

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