Electron capture dissociation of peptides metalated with alkaline-earth metal ions.

Journal of the American Society for Mass Spectrometry
Y M Eva FungT-W Dominic Chan

Abstract

The possible use of divalent alkaline-earth metal ions, including Mg2+, Ca2+, Sr2+, and Ba2+, as charge carrier for electron capture dissociation of peptides was investigated. Model peptides of RGGGVGGGR and NGGGWGGGN were used to simplify the interpretation of spectral information. It was demonstrated that useful electron capture dissociation (ECD) tandem mass spectra of these metalated peptides could be generated. Interestingly, peptides metalated with different alkaline-earth metal ions generated very similar ECD tandem mass spectra. Metalated c-ions and z-ions were the predominant fragment ions. Only Mg2+-metalated peptides gave somewhat different results. Some nonmetalated c-ions were observed from ECD of [RGGGVGGGR + Mg]2+ but not from [NGGGWGGGN + Mg]2+. Together with some ab initio calculations, it was established that the bound metal ions might activate the acidity of the amide hydrogen. With the presence of high proton affinity moiety, such as N-terminal amino group and/or side chain of the arginine residues, the metalated peptide ions could exist predominantly in their zwitterion forms, in which one or two backbone amide group(s) was deprotonated and the high proton affinity functional group(s) was protonated. It was...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·R N Hayes, M L Gross
Jan 15, 1999·Journal of the American Society for Mass Spectrometry·K W Fong, T W Chan
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Siu Kwan SzeFred W McLafferty
Mar 23, 2002·Journal of the American Society for Mass Spectrometry·Helen J CooperAlan G Marshall
Apr 23, 2002·Rapid Communications in Mass Spectrometry : RCM·Magnus PalmbladPer Håkansson
Dec 18, 2002·Journal of the American Society for Mass Spectrometry·T W Dominic Chan, W H Herman Ip
Dec 18, 2002·Journal of the American Society for Mass Spectrometry·Ziqiang Guan
Dec 31, 2002·Journal of the American Society for Mass Spectrometry·Kristina HåkanssonRichard A J O'Hair
Mar 13, 2003·Journal of the American Chemical Society·Frantisek Turecek, Erik A Syrstad
May 28, 2003·Mass Spectrometry Reviews·Roman A Zubarev
Jun 5, 2003·Journal of the American Society for Mass Spectrometry·Ziqiang GuanRay Bakhtiar
Jul 17, 2003·Journal of the American Chemical Society·Nancy LeymariePeter B O'Connor
Apr 15, 2004·Analytical Chemistry·Anthony T IavaroneEvan R Williams
Jul 30, 2004·Rapid Communications in Mass Spectrometry : RCM·Youri O TsybinPer Håkansson
Dec 14, 2004·Journal of the American Society for Mass Spectrometry·Romulus MihalcaRon M A Heeren
Feb 8, 2005·Journal of the American Society for Mass Spectrometry·Erik A Syrstad, Frantisek Turecek
Jul 19, 2005·Journal of the American Society for Mass Spectrometry·Y M Eva Fung, T-W Dominic Chan

❮ Previous
Next ❯

Citations

Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Xiangfeng ChenTak Wah Dominic Chan
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Xiangfeng ChenT-W Dominic Chan
Dec 14, 2011·Journal of the American Society for Mass Spectrometry·Jia Dong, Richard W Vachet
Jan 4, 2013·Journal of the American Society for Mass Spectrometry·Tawnya G FlickEvan R Williams
Sep 6, 2006·Journal of the American Society for Mass Spectrometry·Haichuan Liu, Kristina Håkansson
Nov 23, 2006·Journal of the American Society for Mass Spectrometry·Jace W JonesFrantisek Turecek
Jul 17, 2007·Journal of the American Society for Mass Spectrometry·K F MedzihradszkyA L Burlingame
Oct 2, 2007·Journal of the American Society for Mass Spectrometry·Haichuan Liu, Kristina Håkansson
Nov 21, 2007·Journal of the American Society for Mass Spectrometry·Arugadoss DevakumarJames P Reilly
Aug 5, 2009·Journal of the American Society for Mass Spectrometry·Camilla Skinnerup JensenSteen Brøndsted Nielsen
Dec 17, 2009·Journal of the American Society for Mass Spectrometry·Malgorzata A KaczorowskaHelen J Cooper
May 9, 2013·Chemical Reviews·František Tureček, Ryan R Julian
Feb 4, 2014·Journal of the American Society for Mass Spectrometry·Rebecca H WillsPeter B O'Connor
Mar 29, 2014·Journal of the American Society for Mass Spectrometry·Daiki AsakawaYoshinao Wada
May 23, 2014·Journal of the American Society for Mass Spectrometry·Yiqun HuangCheng Lin
Feb 13, 2016·Rapid Communications in Mass Spectrometry : RCM·Xiangfeng ChenT-W Dominic Chan
Nov 30, 2011·Journal of Mass Spectrometry : JMS·Heather M WatsonCarolyn J Cassady
Aug 13, 2015·Journal of the American Society for Mass Spectrometry·Valery G VoinovDouglas F Barofsky
Mar 23, 2015·The Journal of Chemical Physics·Bruno BellinaVitaly V Kresin
Oct 19, 2014·Journal of the American Society for Mass Spectrometry·Juan WeiPeter B O'Connor
Jun 14, 2016·Journal of the American Society for Mass Spectrometry·Juliette J Commodore, Carolyn J Cassady
Feb 9, 2017·Journal of Mass Spectrometry : JMS·Juliette J Commodore, Carolyn J Cassady
Sep 18, 2018·Journal of Mass Spectrometry : JMS·Juliette J Commodore, Carolyn J Cassady
Feb 28, 2007·Journal of Mass Spectrometry : JMS
Apr 9, 2008·The Journal of Physical Chemistry. B·Cristina TrujilloJean-Yves Salpin
Nov 6, 2020·Chemical Reviews·Eric GloaguenMarkus Gerhards

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of the American Society for Mass Spectrometry
Haichuan Liu, Kristina Håkansson
Journal of the American Society for Mass Spectrometry
Erik A Syrstad, Frantisek Turecek
© 2022 Meta ULC. All rights reserved