Electron microscope based X-ray microanalysis on bioaccumulation of heavy metals and neural degeneration in mudskipper [Pseudapocryptes lanceolatus

Journal of Microscopy and Ultrastructure
Swaraj K Sarkar, Subrata K De

Abstract

The bioaccumulation of heavy metals and its probable cytological consequences in ciliated olfactory sensory receptor neuron (OSN) of two different groups of Pseudapocryptes lanceolatus has been studied using X-ray microanalysis in transmission electron microscopy (TEM-EDX) [i.e., Group I, collected near Kanchrapara (22.56°N 88.26°E) and Group II, collected near Tribeni (22.99°N 88.40°E) of West Bengal, India]. The ciliated OSN is a bipolar neuron and possesses a prolonged dendron with four to six cilia at the olfactory knob, perikaryon, and axon. Excess accumulation of copper (94.50%) and iron (83.81%) was noted under TEM-EDX in the cytoplasm of the olfactory knob as well as nucleoplasm of ciliated OSNs in P. lanceolatus (Group II). The degenerating ciliated OSNs show distinct features of lysis of the plasma membrane at the olfactory knob, disintegration of cytoskeletal structures in perinuclear cytoplasm and axoplasm, and fragmented chromatin fibers with granules (diameter, 20-30 nm) in the nucleoplasm. Crowding of acetylcholinesterase-positive vesicles (diameter:, 30-40 nm) at the terminal part of the axoplasm was related to accumulation of heavy metals in degenerating ciliated OSNs of P. lanceolatus (Group II). The recorded ...Continue Reading

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Methods Mentioned

BETA
transmission electron microscopy
X-ray

Software Mentioned

iTEM

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