PMID: 5920196Apr 1, 1966

Electron microscope studies on the active accumulation of Sr++ by rat-liver mitochondria

The Journal of Cell Biology
J W Greenawalt, E Carafoli

Abstract

Electron-opaque granules are deposited in isolated rat-liver mitochondria concomitant with the energy-linked accumulation of Sr(++) by these organelles. High temperature microincineration (600 degrees C) of thin sections of mitochondria containing different amounts of Sr(++) shows that a clear qualitative correlation exists between the number of inorganic residues remaining after incineration and the amount of Sr(++) translocated into the mitochondria. By loading the mitochondria with consecutive pulses of small amounts of Sr(++) ("multiple-pulse" loading), very early stages of granule formation can be detected; the first detectable deposits are seen closely associated with the cristae. The evidence presented supports the hypothesis that mineral deposition following or during the in vitro accumulation of ions by mitochondria occurs, at least initially, at sites on these membranes and not as nonspecific precipitates in the mitochondrial matrix. The large number of electron-opaque deposits (100 to 200) seen in single thin sections of individual mitochondria having accumulated intermediate levels of Sr(++) clearly exceeds the number of normal dense granules in rat-liver mitochondria, indicating that the normal matrix granules per ...Continue Reading

References

Jul 8, 1965·Biochimica Et Biophysica Acta·A I Caplan, E Carafoli
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT
Nov 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·G P BrierleyD E Green
Jan 4, 1965·Biochimica Et Biophysica Acta·E CarafoliA L Lehninger
Mar 1, 1955·The Journal of Biophysical and Biochemical Cytology·R F WITTERM A COTTONE
Dec 1, 1961·The Journal of Biophysical and Biochemical Cytology·M J KARNOVSKY

Citations

Jun 1, 1984·Neurochemical Research·S Ochs, R A Jersild
Nov 24, 1975·Calcified Tissue Research·C Gay, H Schraer
Jan 1, 1986·Journal of Ultrastructure and Molecular Structure Research·R C HertsensW A Jacob
Jun 1, 1975·Journal of Ultrastructure Research·E BonillaB Aldover
Jan 22, 1998·Free Radical Biology & Medicine·H NakaharaL Packer
Feb 1, 1969·The Journal of Cell Biology·I Pasquali-RonchettiE Carafoli
Nov 1, 1972·The Journal of Cell Biology·W Hohman, H Schraer
Oct 1, 1973·The Journal of Cell Biology·E BonucciV Marinozzi
Jan 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amit K ChouhanGregory T Macleod
Feb 1, 1973·Experimental Cell Research·R Ramirez-MitchellR H Wilson
Apr 1, 1968·Journal of Ultrastructure Research·U MuscatelloA Barasa
Jul 1, 1967·Journal of Ultrastructure Research·R L Wood
Sep 15, 2014·Molecular and Biochemical Parasitology·Roberto DocampoGuozhong Huang
Apr 14, 2010·Biochimica Et Biophysica Acta·E Carafoli
Nov 1, 1968·Acta Physiologica Scandinavica·B AbergJ A Nyberg
Sep 1, 1980·British Journal of Haematology·J A GrassoA L Sullivan
Jan 1, 1980·Acta Neuropathologica·E BonillaS Di Mauro

Related Concepts

Liver
Electron Microscopy
Mitochondria
Strontium

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