Electron microscope studies on the intracellular growth of PL-1 phage of Lactobacillus casei

Microbiology and Immunology
K WatanabeA Kuroiwa

Abstract

Ultrathin sections of the cells of Lactobacillus casei infected with or without PL-1 phages were observed by the rapid-freezing and substitution-fixation method. Phage-head-like particles were first observed in the nuclear region. The region was seen more widely dispersed in the cytoplasm than that observed by the conventional chemical fixation method. The features of cells just broken open by the infected phages were observed by the sedimentation method devised by us. The bursting occurred in more than one place in the cells with liberation of progeny phages.

References

Jun 1, 1979·The Journal of General Virology·K H LundströmK Lounatmaa
Jul 1, 1967·Scientific American·W B Wood, R S Edgar
Feb 1, 1984·Journal of General Microbiology·K WatanabeY Nakashima
Jan 1, 1981·Intervirology·T SozziM Smiley

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Citations

Jan 1, 1992·Electron Microscopy Reviews·L L Graham

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