Electron microscopic analysis of Drosophila midline glia during embryogenesis and larval development using beta-galactosidase expression as endogenous cell marker

Microscopy Research and Technique
A StollewerkR Cantera

Abstract

To thoroughly study developmental problems it is often desirable to identify specific cells at the resolution of the electron microscope (TEM). Specific antibodies, and immunogold and other antibody labelling techniques can be successfully used with the TEM. But for these techniques to be successful there must be substantial adjustments for each antibody and tissue analyzed. To develop a more generally applicable labelling method we took advantage of the enhancer trap technique in Drosophila. Enhancer trap fly strains show cell- and/or tissue-specific beta-galactosidase expression which can be visualized by a simple X-gal staining procedure. To combine the power of the enhancer trap approach with electron microscopy, we have improved the fixation and staining conditions, which allow detection of X-gal crystals (by TEM) and thus provide precise information on ultrastructural morphology. We have tested our technique using the well-known midline glial cells and examined these cells between late embryonic and pupal developmental stages. The four embryonic midline glial cells found in each neuromere reside ventrally and dorsally to the midline of the neuropile and are closely associated with unpaired neurons, major commissures, and ...Continue Reading

References

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Citations

Feb 4, 2016·Nature·Darren J BakerJan M van Deursen
Feb 26, 2020·Developmental Neurobiology·Jonas BitternChristian Klämbt
Feb 7, 2012·Developmental Biology·Viktoria LinneAngelika Stollewerk
Feb 1, 1997·Mechanisms of Development·H ScholzC Klämbt
Jun 1, 1997·Mechanisms of Development·H ScholzC Klämbt
Sep 11, 2002·Developmental Biology·Jan PielageChristian Klämbt
Apr 5, 2003·Mechanisms of Development·Kay GiesenChristian Klämbt
Dec 29, 2005·Developmental Biology·Ariel D Chipman, Angelika Stollewerk
Dec 21, 2006·Differentiation; Research in Biological Diversity·Roxane H SchröterRenate Renkawitz-Pohl

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