PMID: 6112213Jan 1, 1981Paper

Electron microscopic cytochemical localization of nucleoside phosphatases in normal and chronic granulocytic leukaemic human neutrophils

The Histochemical Journal
P D WilsonT J Peters

Abstract

Using electron microscope cytochemistry and cells separated on Ficoll-Hypaque, Mg2+-dependent ATPase, ADPase and 5'-nucleotidase were predominantly localized as ectoenzymes on normal human granulocytes. Large deposits of ATPase final reaction product and more finely granular deposits of 5'-nucleotidase final reaction product were firmly attached to the outer surface of cell plasma membranes. The final reaction product from ecto-ADPase was, however, only loosely associated with the plasma membrane. In addition, finer deposits of ADPase final reaction product were seen in specific granules and in background cytoplasm. No nucleotidase phosphatase activity was localized to the alkaline phosphatase-containing granules (phosphasomes) recently described by Rustin et al. In granulocytes from patients with chronic granulocytic leukaemia, ecto-ATPase had a patchy distribution on the plasma membranes. There was considerable heterogeneity between cells with regard to ADPase and 5'-nucleotidase localization. In some cells, ADPase was seen only at both site, while in some cells no activity was detected. 5'-Nucleotidase localization was normal in some cells but lacking from many. No correlation was found between enzyme heterogeneity and the d...Continue Reading

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Citations

Sep 1, 1989·Circulation Research·S B Coade, J D Pearson
Aug 29, 2000·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·G Lundborg
Oct 26, 2006·Brain Injury : [BI]·Anna CantagalloLuigi Tesio
Jan 1, 1998·Occupational Therapy in Health Care·A Benevento
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gianluca FossatiSteven W Edwards
Mar 23, 2021·Frontiers in Immunology·Michele Fresneda AlarconHelen Louise Wright
Aug 4, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·K B RajaT J Peters
Apr 11, 1985·Biochimica Et Biophysica Acta·R T O'Donnell, B R Andersen

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