PMID: 61692Jan 1, 1976Paper

Electron microscopic demonstration of hyaluronidase sensible proteoglycans at the presynaptic area in mouse cerebellar cortex

Acta histochemica
H Castejón, O J Castejón

Abstract

By application of electron cytochemical techniques to cerebellar tissue, the presence of proteoglycans was demonstrated at the axoplasmic matrix of mossy fiber endings. Blocks of glutaraldehyde (G) fixed mouse cerebellum were processed according to the following procedures: a) Some pieces of tissue were post-fixed in osmium tetroxide, dehydrated by ethanol and embedded in araldite. b) Other pieces were sectioned to 30 mum thick and then immersed in Alcian blue solution pH = 2.7 followed by osmium tetroxide fixation, dehydrated and embedded in araldite (GABOUL procedure). c) Parallel slices of (b) previous to Alcian blue immersion were washed and incubated in either methanol-HCl, neuraminidase, ribonuclease or testicular hyaluronidase with their respective controls. d) Other blocks of G fixed tissue without any other treatment and fixation were dehydrated and embedded in araldite. Ultrathin sections of a, b and c were doubly stained with uranyl acetate and lead citrate while ultrathin sections of (d) were stained with the osmium coordination compound Os-DMEDA. The electron microscopic study revealed at the presynaptic axoplasm of mossy fiber rosettes, the presence of a GABOUL and Os-DMEDA positive electron dense material surroun...Continue Reading

References

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Citations

Jan 1, 1986·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M V Alvarado, H V Castejon
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R K MargolisR U Margolis
Oct 27, 2009·Neurochemistry International·Carine ClerenM Flint Beal
Jan 1, 1984·Journal of Neuroscience Research·A C Grovas, K S O'Shea
Dec 20, 1976·Biochemical and Biophysical Research Communications·R U MargolisR K Margolis

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