PMID: 9185669Jun 1, 1997Paper

Electron microscopic evidence for microglial phagocytic activity and cholinergic cell death after administration of the immunotoxin 192IgG-saporin in rat

Journal of Neuroscience Research
G SeegerK Brauer

Abstract

192IgG-saporin represents a novel cholinergic immunotoxin which selectively and specifically destroys cholinergic cells in rat basal forebrain. Activated microglial cells are known to play an important role in phagocytosis in regions of neuronal loss. To study the immunotoxin-induced phagocytic events in the basal forebrain activated microglial cells were visualized by lectin cytochemistry using Griffonia simplicifolia agglutinin and analyzed by electron microscopy. Three and 7 days following an intracerebro-ventricular injection of 4 microg 192IgG-saporin, increased numbers of activated microglial cells were observed at both survival times, but the number was strikingly increased at day 7 postlesion. Three days after immunotoxin application microglial cells displayed features similar to those of resting microglia. Only translucent vacuole-like hollows were found intracellularly beneath the plasma membrane of microglial cells and in the adjoining extracellular space. Most neurons in the vicinity of microglial cells did not show any signs of degeneration. However, 7 days after injection of the immunotoxin microglial cells revealed different stages of phagocytosis. The majority of microglial cells were localized in perineuronal p...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Nov 8, 1992·The Journal of Comparative Neurology·T NaumannM Frotscher
Jan 1, 1992·Brain Research. Brain Research Reviews·W E Thomas
Nov 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W J Streit
Sep 15, 1989·The Journal of Comparative Neurology·K Ashwell
Feb 8, 1988·The Journal of Comparative Neurology·W J Streit, G W Kreutzberg
Mar 10, 1988·Neuroscience Letters·M B GraeberG W Kreutzberg
Apr 1, 1987·Journal of Neurocytology·W J Streit, G W Kreutzberg
Jan 1, 1982·Cell and Tissue Research·Y Murabe, Y Sano
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D E MaddoxI J Goldstein
Jul 30, 1982·Science·R T BartusA S Lippa
Dec 1, 1994·Trends in Pharmacological Sciences·P EikelenboomD Allsop
Apr 1, 1994·Neuropathology and Applied Neurobiology·M T GiordanaD Schiffer
Jan 1, 1994·Journal of Neuropathology and Experimental Neurology·A A BookJ B Schweitzer
Jun 11, 1993·Neuroscience Letters·M SawadaT Marunouchi
Jul 1, 1993·Journal of Chemical Neuroanatomy·W J Streit
Aug 1, 1996·Trends in Neurosciences·G W Kreutzberg

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Citations

Oct 19, 2017·Biochemistry. Biokhimii︠a︡·N V GulyaevaN A Stefanova
Jul 7, 1999·Neuroscience and Biobehavioral Reviews·E Gahtan, J B Overmier

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