PMID: 4294244Dec 1, 1967Paper

Electron microscopic examination of subcellular fractions. II. Quantitative analysis of the mitochondrial population isolated from rat liver

The Journal of Cell Biology
P Baudhuin, J Berthet

Abstract

Large granule fractions, containing about 80% of the cytochrome oxidase of the tissue, were isolated from rat liver and used to prepare thin pellicles of packed particles which were submitted to quantitative electron microscopic examination. Various parameters describing the mitochondrial population were determined by measuring the size and number of mitochondrial profiles in sections, and the ratio of the inner to the outer membrane area. The mean particle radius and volume were found to be respectively 0.38 micro and 0.29 micro(3); the average areas per mitochondrion were 2 and 5 micro(2) for the outer and inner membranes respectively. On the basis of the cytochrome oxidase activity recovered in the particulate fractions, the results were extrapolated to the whole liver, and it was concluded that rat liver contains about 5.10(11) mitochondria per gram; this corresponds to a volume of 0.14 ml/g and to an area of 2.5 and 1 m(2)/g for the inner and outer membranes respectively. The validity and the accuracy of these determinations is discussed and the results are compared to the information which has been obtained by independent methods or by other investigators.

References

Jan 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·U Glas, G F Bahr
Jan 1, 1966·Experimental Cell Research·G F BahrU Glas
Jul 1, 1966·The Journal of Cell Biology·E R WeibelW F Scherle
Jan 1, 1964·Journal of Theoretical Biology·C De Duve
Jan 1, 1966·Zeitschrift für Zellforschung und mikroskopische Anatomie·F ClementiM N Sheridan
Aug 1, 1967·The Journal of Cell Biology·J Berthet, P Baudhuin
Dec 1, 1962·The Journal of Cell Biology·G F BAHR, E ZEITLER

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Citations

Feb 15, 1979·Cell and Tissue Research·L CieciuraW Klitończyk
Jan 1, 1973·Protoplasma·J Bielawski, B Kwinto
Mar 1, 1976·Developmental Biology·L Pikó, L Matsumoto
Apr 1, 1985·Cell Differentiation·E Marinos
Aug 1, 1981·Molecular and Cellular Endocrinology·M C ManyS Haumont
Feb 27, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·A GosslauP P Jaros
Jul 1, 1970·The Journal of Cell Biology·J N Loeb, D V Kimberg
Nov 1, 1970·The Journal of Cell Biology·W J Larsen
Jul 1, 1971·The Journal of Cell Biology·C de Duve
Mar 1, 1972·The Journal of Cell Biology·N YagoH Kurokawa
Feb 1, 1980·The Journal of Cell Biology·H Mori, A K Christensen
May 1, 1973·Journal of Microscopy·D Froesch
Apr 1, 1974·Journal of Microscopy·E R Weibel
Sep 13, 1971·European Journal of Biochemistry·R WattiauxM F Ronveaux-Dupal
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·C LavoieJ J Bergeron
Sep 1, 1982·Critical Reviews in Toxicology·N H Diemer
Jan 1, 1969·Tissue & Cell·G G Laties, T Treffry
Nov 1, 1969·Journal of Ultrastructure Research·T Barnard, O Lindberg
Dec 1, 1973·Analytical Biochemistry·L ChurchillC W Cotman
Nov 2, 1979·Biochimica Et Biophysica Acta·C W Campbell
Oct 1, 1984·The Anatomical Record·S KanamuraM Asada-Kubota
Jul 1, 1973·Journal of Ultrastructure Research·K E Rasmussen

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