PMID: 3745912Oct 1, 1986Paper

Electron microscopic immunocytochemistry. Silver enhancement of colloidal gold marker allows double labeling with the same primary antibody

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
K BienzL Pasamontes

Abstract

Electron microscopic sections, immunocytochemically labeled with colloidal gold, can be prepared for double labeling by applying the "EM-silver enhancement" procedure. This method, a photographic, so-called physical, development, increases the size of the gold marker to a predeterminable value and thereby inactivates the anti-species antibody present on the gold grain, thus allowing the labeling of a second antigen with antibody raised in the same species.

Citations

Aug 26, 1998·Microscopy Research and Technique·W Baschong, Y D Stierhof
Jun 1, 1994·Molecular Biotechnology·D Egger, K Bienz
May 9, 2009·Histochemistry and Cell Biology·Elena BogdanovicDaniel J Dumont
Apr 1, 1993·Journal of Neuroscience Methods·D W Smith, T A Day
Apr 1, 1996·Micron : the International Research and Review Journal for Microscopy·P J Koeck, K R Leonard
Jan 1, 1991·The European Journal of Neuroscience·Patrizia MorinoPeter Streit
Jan 1, 1990·The Histochemical Journal·E De HarvenH Christensen
Jun 5, 2007·Colloids and Surfaces. B, Biointerfaces·Qun Huo
Nov 29, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F WangS Yonezawa
Feb 22, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H YiS Hersch
Mar 22, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H Sawada, M Esaki
Dec 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Nigel P GoodeAndrew F Mooney
Jan 28, 2006·The Journal of Comparative Neurology·Anne-Marie HaeberléYannick Bailly

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.