ELECTRON MICROSCOPIC LOCALIZATION OF MACROMOLECULES ON MEMBRANE SURFACES

Annals of the New York Academy of Sciences
G L Nicolson, S J Singer

Abstract

Ferritin-conjugated antibodies and ferritin-conjugated plant agglutinins can be used as specific stains to localize macromolecular antigens and specific saccharide residues, respectively, on membranes. In conjunction with a new procedure for preparing membrane specimens by cytolysis at an air-water interface, specific ferritin-conjugated antibodies have been used to determine the distribution of the Rh0(D) antigen on human erythrocyte membranes, and of the H-2 histocompatibility alloantigens on murine erythrocytes. Over a period of time these distributions are random, lending support to the lipid-globular protein mosaic model of membrane structure. With ferritin-conjugated plant agglutinins, it has been found, for several types of saccharide residues and several varieties of plasma membranes, that the membrane-bound oligosaccharides are exclusively located on the outer surfaces of the membranes; none is found on the inner cytoplasmic surface. These results strongly suggest that the glycoproteins of membranes do not rotate from the outer to the inner surface at a significant rate under physiological conditions.

References

May 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·G L Nicolson, S J Singer
Jan 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J D McLean, S J Singer
Mar 30, 1971·Biochemistry·R D Kornberg, H M McConnell
Sep 1, 1970·Clinical Toxicology·C Y Lee
Feb 18, 1972·Science·S J Singer, G L Nicolson
Mar 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·A ShimadaS G Nathenson
Jul 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·T Shirai, R C Mellors
Feb 1, 1959·The Journal of Clinical Investigation·S P MASOUREDIS
Nov 1, 1964·The Journal of Cell Biology·R E LEE, J D FELDMAN

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Citations

Dec 1, 1972·The American Journal of Anatomy·R YanagimachiG L Nicolson

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