Electron microscopic structure of agrin and mapping of its binding site in laminin-1

The EMBO Journal
A J DenzerM A Rüegg

Abstract

Agrin is a large, multidomain heparan sulfate proteoglycan that is associated with basement membranes of several tissues. Particular splice variants of agrin are essential for the formation of synaptic structures at the neuromuscular junction. The binding of agrin to laminin appears to be required for its localization to synaptic basal lamina and other basement membranes. Here, electron microscopy was used to determine the structure of agrin and to localize its binding site in laminin-1. Agrin appears as an approximately 95 nm long particle that consists of a globular, N-terminal laminin-binding domain, a central rod predominantly formed by the follistatin-like domains and three globular, C-terminal laminin G-like domains. In a few cases, heparan sulfate glycosaminoglycan chains were seen emerging from the central portion of the core protein. Moreover, we show that agrin binds to the central region of the three-stranded, coiled-coil oligomerization domain in the long arm of laminin-1, which mediates subunit assembly of the native laminin molecule. In summary, our data show for the first time a protein-protein interaction of the extracellular matrix that involves a coiled-coil domain, and they assign a novel role to this domain ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Apr 1, 1992·Neuron·K W TsimU J McMahan
Oct 1, 1992·Current Opinion in Cell Biology·U J McMahanG Escher
May 1, 1991·Neuron·F RuppR H Scheller
Nov 15, 1991·European Journal of Biochemistry·M GerlR Timpl
Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·U J McMahan
Aug 1, 1990·Trends in Biochemical Sciences·N Jentoft
Dec 1, 1987·The Journal of Cell Biology·N E ReistU J McMahan
Dec 1, 1987·The Journal of Cell Biology·R M NitkinU J McMahan
Jan 1, 1987·Methods in Enzymology·R TimplS Fujiwara
Mar 1, 1982·European Journal of Biochemistry·U OttR Timpl
Jun 30, 1995·Journal of Molecular Biology·R A KammererJ Engel
Jul 1, 1995·Protein Science : a Publication of the Protein Society·P Bork, L Patthy
Feb 1, 1993·Trends in Neurosciences·L Patthy, K Nikolics
Mar 1, 1995·Trends in Biochemical Sciences·P BorkJ McEntyre
Oct 1, 1994·Current Opinion in Cell Biology·P D Yurchenco, J J O'Rear
Feb 17, 1995·The Journal of Biological Chemistry·G TsenG J Cole
Apr 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·R E BurgesonJ Sanes
Jul 1, 1994·Neuron·J SugiyamaZ W Hall
Aug 1, 1993·Developmental Biology·W S ThomasM A Smith
May 17, 1996·Cell·D J GlassG D Yancopoulos
Feb 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Timpl, J C Brown
Oct 1, 1996·Current Opinion in Cell Biology·R Timpl
Oct 1, 1996·Current Opinion in Cell Biology·M D Henry, K P Campbell
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·G JonesH R Brenner

❮ Previous
Next ❯

Citations

Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Colognato, P D Yurchenco
May 26, 2009·Molecules and Cells·Renato V IozzoAlexander Nyström
Mar 14, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·M A Ruegg
Feb 20, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Erhard Hohenester, Jürgen Engel
Jun 12, 2003·Neuromuscular Disorders : NMD·Alexander EusebioMarkus A Ruegg
Jul 26, 2007·Biochemistry·Christine O SallumAndrei T Alexandrescu
Sep 22, 1999·European Journal of Biochemistry·W Hoch
Jan 29, 2000·Kidney International·C J RaatsJ H Berden
Nov 27, 2009·The Journal of Biological Chemistry·Elmar PortenStephan Kröger
Feb 5, 2009·The Journal of Biological Chemistry·Karen K McKeePeter D Yurchenco
Sep 10, 2002·Neuroreport·Martin A Smith, Lutz G W Hilgenberg
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Peter D Yurchenco
Feb 4, 2012·Genes & Development·Yinong ZongRongsheng Jin
Dec 4, 2009·Journal of Cell Science·Kevin J HamillJonathan C R Jones
Jan 17, 2002·Journal of Neurobiology·John L BixbyMarkus A Rüegg
Jun 17, 2004·Journal of Neurochemistry·Kristine Baerwald-de la TorreJohn L Bixby
Jul 1, 2009·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·David S Goodsell
Sep 13, 2008·The FEBS Journal·Glynis JohnsonSamuel W Moore
Jul 19, 2005·Trends in Biochemical Sciences·Jörg Stetefeld, Markus A Ruegg
Feb 24, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Renato V Iozzo, Liliana Schaefer
Oct 19, 2012·Cell Adhesion & Migration·Erhard Hohenester, Peter D Yurchenco
May 18, 1999·Experimental Cell Research·S L CotmanG J Cole
Mar 28, 2007·Journal of Biotechnology·Norbert W KolarPieter Swart
Sep 10, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Dorin-Bogdan Borza
Oct 31, 2000·Microscopy Research and Technique·P TunggalM C Ott
Jul 18, 2003·The Journal of Biological Chemistry·C Axel Innis, Marko Hyvönen
Nov 21, 2001·Nature Reviews. Neuroscience·J R Sanes, J W Lichtman
Mar 30, 2001·The Journal of Biological Chemistry·Y SidisH T Keutmann
Aug 28, 2019·The Journal of Comparative Neurology·Geoffroy NoëlHakima Moukhles
Oct 18, 2019·Biomaterials Science·Daniela BarrosAna P Pêgo
Nov 21, 2019·Cells·Lauren Eric Swenarchuk
Sep 17, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·A J GroffenL P van den Heuvel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.