PMID: 3746895May 1, 1986Paper

Electron microscopic study of 5S rRNA crystals from Thermus thermophilus HB8

Journal of Microscopy
K MorikawaS Takemura

Abstract

Several types of crystals were grown from 5S rRNA, which was purified from the highly thermophilic bacterium, Thermus thermophilus HB8. Crystal lattice parameters were determined by X-ray and electron diffraction. One type of crystal was suitable for electron microscopy after staining with uranyl acetate. Two projections derived from micrographs taken at different tilt angles were processed for image analysis. This result enabled us to deduce the arrangement of molecules within the crystal lattice.

References

Dec 14, 1972·Journal of Molecular Biology·J E LadnerB F Clark
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T Pieler, V A Erdmann
Jan 1, 1982·Progress in Biophysics and Molecular Biology·L A AmosP N Unwin
Feb 24, 1984·Nucleic Acids Research·N B Leontis, P B Moore
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT

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Citations

Apr 19, 1990·Biochimica Et Biophysica Acta·G Harauz, D Flannigan
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R S BrownK R Leonard
Jan 6, 1992·Biochimica Et Biophysica Acta·G HarauzD Flannigan

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