PMID: 6972859Mar 31, 1981Paper

Electron microscopical and chemical investigations of the pathological changes of the vitreous body

Documenta Ophthalmologica. Advances in Ophthalmology
U Klemen

Abstract

By means of vitrectomy in vivo investigations of the pathologic changes of the vitreous body including histology and chemical analysis have become feasible. The electron microscopical examinations of the tissue particles gained by means of vitrectomy displayed collagen fibrils which were partly irregular, most of the mesh-work being split up. The periodicity of these fibrils was about 64 nm, similar changes had been observed in human vitreous following perforating injuries, as well as in rabbit vitreous after blood injections. All typical stages of the lysis of red cells and their phagocytosis by large macrophages could be demonstrated. In order to get an accurate chemical analysis of the material resulting from vitrectomy it was first to all necessary to find out a method which enabled us to compare the concentrations of different chemical substances in the material gained by vitrectomy with the content of these substances in the whole vitreous body. Of 16 pairs of human post mortem eyes, enucleated between 3 and 5 hours after death, one eye was submitted to vitrectomy under constant suction- and infusion-pressure and cutting rate, the duration of surgery being different (3-9 min.); in each fellow-eye the whole vitreous body w...Continue Reading

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