PMID: 6170609Sep 1, 1981Paper

Electron microscopical localization of nucleic acids by means of nuclease--gold complexes

The Histochemical Journal
M Bendayan

Abstract

Nucleic acids can be specifically localized at the electron microscope level by means of enzyme--gold complexes. Two enzymes RNAase A and DNAase I were labelled with colloidal gold, and the enzyme--gold complexes obtained applied on thin sections of glutaraldehyde-fixed and Epon-embedded tissues. Using RNAase--gold, the rough endoplasmic reticulum and the nucleolus of different cells appeared densely labelled. With the DNAase--gold the labelling was present over the euchromatin and the mitochondria. The quantitative evaluation, performed on different cellular compartments of the pancreatic acinar cells, confirmed the qualitative observations and ascertained the specificity of the labelling. The application of this technique, for the demonstration of nucleic acids in different tissues, is illustrated.

References

Aug 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L GuesdonS Avrameas
Aug 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E A BayerM Wilchek
Dec 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J RothL Orci
Aug 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J P PetraliL A Sternberger
Jan 1, 1969·International Review of Cytology·E R Weibel
Jan 1, 1980·Progress in Histochemistry and Cytochemistry·G Moyne
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Bendayan
Feb 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M BendayanL Orci

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Citations

Jan 1, 1987·Archives of Virology·S Patterson, B J Verduin
May 26, 2004·BMC Biology·Francisco J IborraPeter R Cook
May 1, 1988·Ultrastructural Pathology·R HolmJ V Johannessen
Jul 1, 1989·Ultrastructural Pathology·K Ishikawa, M Eguchi
Jan 1, 1985·Progress in Histochemistry and Cytochemistry·J A Litwin
Nov 5, 1999·Progress in Histochemistry and Cytochemistry·M Thiry
Jan 8, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Diane Gingras, Moïse Bendayan

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